Molecular analysis of the Cip1/Waf1 (p21) gene in diverse types of human tumors

We have screened for mutations in the Cip1/Wafa gene using Southern blot analysis and the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method in diverse human tumors. Seven of 102 (7%) human tumor samples were identified to have point mutations within the coding regio...

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Published inBiochimica et biophysica acta Vol. 1263; no. 3; pp. 275 - 280
Main Authors Watanabe, Hiroyuki, Fukuchi, Kunihiko, Takagi, Yasushi, Tomoyasu, Shigeru, Tsuruoka, Nobuyoshi, Gomi, Kunihide
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 19.09.1995
Subjects
Base Sequence
Blotting, Southern
Cip1/Wafl (p21) gene
Cyclin-dependent kinase inhibitor
DNA Probes
Gene Rearrangement
Genes, Tumor Suppressor
Humans
Molecular Sequence Data
Mutation - genetics
Neoplasms - genetics
PCR-SSCP
Polymerase Chain Reaction
Tumor suppressor gene
Cyclin-dependent kinase inhibitor
PCR-SSCP
Cip1
Cip1/Wafl (p21) gene
Cdk
Waf1
Sdi1
Tumor suppressor gene
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Summary:We have screened for mutations in the Cip1/Wafa gene using Southern blot analysis and the polymerase chain reaction single-strand conformation polymorphism (PCR-SSCP) method in diverse human tumors. Seven of 102 (7%) human tumor samples were identified to have point mutations within the coding region of the Cip1/Waf1 gene. Two of the seven mutated cases showed gene rearrangements. These results suggest that the frequency of genetic alterations in the Cip1/Waf1 gene is relatively low in comparison with several known tumor suppressor genes.
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ISSN:0167-4781
0006-3002
1879-2634
DOI:10.1016/0167-4781(95)00110-3