Multibiosensor based on enzyme inhibition analysis for determination of different toxic substances

• Published in: Talanta (Oxford) Vol. 55; no. 5; pp. 919 - 927
• Main Authors: Arkhypova, V.N, Dzyadevych, S.V, Soldatkin, A.P, El'skaya, A.V, Jaffrezic-Renault, N, Jaffrezic, H, Martelet, C
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Amsterdam Elsevier B.V 13.12.2001; Oxford Elsevier
• Subjects: Biological and medical sciences; Biosensors; Biotechnology; Electrodes; Enzyme inhibition; Field effect transistors; Fundamental and applied biological sciences. Psychology; Heavy metals; Inhibition analysis; Methods. Procedures. Technologies; Multibiosensor; Multivariate correspondence analysis; Pesticides; Positive ions; Potentiometric sensors; Toxic materials; Toxic substances; Transducers; Various methods and equipments; Inhibition analysis; Multibiosensor; Toxic substances; Multivariate correspondence analysis; Enzyme; Pesticides; Esterases; Urease; Acetylcholinesterase; Multivariate analysis; Potentiometry; Cholinesterase; Carboxylic ester hydrolases; Heavy metal; Ionosensible field effect transistor; Pollutant; Conductometry; Biosensor; Simultaneous measurement; Hydrolases; Poison; Inhibition
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/S0039-9140(01)00495-7

An original concept of an enzyme multibiosensor for determination of toxic substances based on enzyme inhibition analysis has been proposed and its main performances have been analysed. For the development of this multibiosensor, two types of transducers such as potentiometric pH-sensitive field-effect transistors and conductometric thin-films interdigitated electrodes, and three enzymes, namely urease, acetylcholinesterase and butyrylcholinesterase have been used. The experimental data have been treated by multivariate correspondence analysis. A complete procedure for a simultaneous determination of some heavy metal ions and pesticides has been proposed and its advantages have been discussed.