Determination of polarity and bilateral asymmetry in palleal and vascular buds of the ascidian Botryllus schlosseri

• Published in: Developmental biology Vol. 46; no. 1; pp. 79 - 87
• Main Authors: Sabbadin, A., Zaniolo, G., Majone, F.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.09.1975
• Subjects: Animals; Morphogenesis; Reproduction, Asexual; Urochordata - growth & development
• ISSN: 0012-1606; 1095-564X
• DOI: 10.1016/0012-1606(75)90088-3

Reversal of the bilateral asymmetry of the zooids was induced in a series of colonies of Botryllus schlosseri. Palleal buds from colonies with normal or reversed bilateral asymmetry were isolated in the early stages from the parental zooids and cultured in the vascularized tunic of the same colony or of another colony with opposite asymmetry. Vascular budding was induced in colonies with either type of asymmetry. The bud polarity was shown to depend on the vascularization; the test vessel entering the isolated palleal bud always causes the entrance point to become the posterior end of the developing zooid. On the contrary, the bilateral asymmetric type is predetermined in the bud primordium; the isolated palleal buds develop the type of asymmetry of their parents, even when grafted in the test of a colony with opposite asymmetry. Since the same was also true of the vascular buds, it is concluded that the information for the kind of bilateral asymmetry to be developed is conveyed by the epidermal envelope of the bud. The epidermis of the parental zooids influences the palleal buds, whereas the wall of the test vessels, epidermal extrusions of the zooids, influences the vascular buds.