Influence of the difference in refractive index on the interface of an object and the surroundings in near-infrared fluorescence tomography

The refraction of fluorescence from the inside of a sample at the surface results in fluctuations in fluorescence computed tomography (CT). We evaluated the influence of the difference in refractive index (RI) between the sample body and the surroundings on fluorescence CT results. The brightest flu...

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Bibliographic Details
Published inApplied optics. Optical technology and biomedical optics Vol. 61; no. 2; p. 638
Main Authors Takematsu, Maho, Umezawa, Masakazu, Sera, Toshihiro, Kitagawa, Yuichi, Kurahashi, Hiroyuki, Yamada, Shuta, Okubo, Kyohei, Kamimura, Masao, Yokota, Hideo, Soga, Kohei
Format Journal Article
LanguageEnglish
Published United States 10.01.2022
Subjects
Refraction, Ocular
Refractometry
Tomography
Tomography, X-Ray Computed
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Summary:The refraction of fluorescence from the inside of a sample at the surface results in fluctuations in fluorescence computed tomography (CT). We evaluated the influence of the difference in refractive index (RI) between the sample body and the surroundings on fluorescence CT results. The brightest fluorescent point is away from the correct point on the tomograms owing to the refraction. The speculated position is determined as the exact point if the RI ratio ranges between 0.97 and 1.03 by immersing the body in an RI matching liquid. The results can help in experimental settings of fluorescence CT for acquiring three-dimensional positional information.
ISSN:2155-3165
DOI:10.1364/ao.445357