HO-1 upregulation promotes mitophagy-dependent ferroptosis in PM2.5-exposed hippocampal neurons

• Published in: Ecotoxicology and environmental safety Vol. 277; p. 116314
• Main Authors: Li, Xiaolan, Ran, Qin, He, Xiang, Peng, Dan, Xiong, Anying, Jiang, Manling, Zhang, Lei, Wang, Junyi, Bai, Lingling, Liu, Shengbin, Li, Shiyue, Sun, Baoqing, Li, Guoping
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.06.2024; Elsevier
• Subjects: Air Pollutants - toxicity; Animals; Ferroptosis; Ferroptosis - drug effects; Heme Oxygenase-1 - genetics; Heme Oxygenase-1 - metabolism; Hippocampus - drug effects; Hippocampus - pathology; HO-1; Male; Membrane Proteins; Mice; Mice, Inbred C57BL; Mitophagy; Mitophagy - drug effects; Neurons - drug effects; Neurons - pathology; Neurotoxicity; Particulate Matter - toxicity; PM2.5; Up-Regulation - drug effects; Ferroptosis; Neurotoxicity; HO-1; Mitophagy; PM2.5
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2024.116314

Fine particulate matter (PM2.5) has been extensively implicated in the pathogenesis of neurodevelopmental disorders, but the underlying mechanism remains unclear. Recent studies have revealed that PM2.5 plays a role in regulating iron metabolism and redox homeostasis in the brain, which is closely associated with ferroptosis. In this study, the role and underlying mechanism of ferroptosis in PM2.5-induced neurotoxicity were investigated in mice, primary hippocampal neurons, and HT22 cells. Our findings demonstrated that exposure to PM2.5 could induce abnormal behaviors, neuroinflammation, and neuronal loss in the hippocampus of mice. These effects may be attributed to ferroptosis induced by PM2.5 exposure in hippocampal neurons. RNA-seq analysis revealed that the upregulation of iron metabolism-related protein Heme Oxygenase 1 (HO-1) and the activation of mitophagy might play key roles in PM2.5-induced ferroptosis in HT22 cells. Subsequent in vitro experiments showed that PM2.5 exposure significantly upregulated HO-1 in primary hippocampal neurons and HT22 cells. Moreover, PM2.5 exposure activated mitophagy in HT22 cells, leading to the loss of mitochondrial membrane potential, alterations in the expression of autophagy-related proteins LC3, P62, and mTOR, as well as an increase in mitophagy-related protein PINK1 and PARKIN. As a heme-degradation enzyme, the upregulation of HO-1 promotes the release of excess iron, genetically inhibiting the upregulation of HO-1 in HT22 cells could prevent both PM2.5-induced mitophagy and ferroptosis. Furthermore, pharmacological inhibition of mitophagy in HT22 cells reduced levels of ferrous ions and lipid peroxides, thereby preventing ferroptosis. Collectively, this study demonstrates that HO-1 mediates PM2.5-induced mitophagy-dependent ferroptosis in hippocampal neurons, and inhibiting mitophagy or ferroptosis may be a key therapeutic target to ameliorate neurotoxicity following PM2.5 exposure. •PM2.5 exposure induced ferroptosis in hippocampal neurons.•Transcriptome analysis revealed the iron metabolism imbalance and autophagy activation.•The activation of mitophagy contributed to the process of ferroptosis.•HO-1 was identified as the key molecule in regulation of mitophagy-dependent ferroptosis.