Polypyrrole-Modified Nylon 6 Nanofibers as Adsorbent for the Extraction of Two β-Lactam Antibiotics in Water Followed by Determination with Capillary Electrophoresis

• Published in: Molecules (Basel, Switzerland) Vol. 24; no. 12; p. 2198
• Main Authors: Li, Xinghua, Miao, Junjie, Yin, Zhendong, Xu, Xiangdong, Shi, Hongmei
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 12.06.2019; MDPI
• Subjects: Adsorbents; Adsorption; Amides; Antibiotic resistance; Antibiotics; Bacteria; Bacterial diseases; Borax; Buffer solutions; Capillary electrophoresis; CE-DAD; determination; Efficiency; Electric potential; Electrophoresis; Gram-negative bacteria; Gram-positive bacteria; Nanofibers; Nanomaterials; Nylon 6; Optimization; PPy-PA6-NFsM; Separation; Sodium channels (voltage-gated); Sodium dodecyl sulfate; solid phase extraction; Voltage; β-Lactam antibiotics; β-lactam residues; β-lactam residues; CE-DAD; determination; solid phase extraction; PPy-PA6-NFsM
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules24122198

A solid phase membrane adsorbent-a nylon 6 nanofibers membrane coated by polypyrrole (PPy-PA6-NFsM)-was firstly synthesized and used for extraction of two β-lactam antibiotics (oxacillin and cloxacillin) in urban river water. Then the analytes were detected by capillary electrophoresis with a diode array detector (CE-DAD). The synthesized nanofibers membrane was characterized by scanning electron microscopy and a Fourier transform infrared spectrometer. The experimental conditions were optimized, including the amount used of PPy-PA6-NFsM, pH of the sample solutions, adsorption volume, and desorption conditions. Under the optimal extraction and separation conditions, the detection limits were found to be 2.0 ng/mL for both oxacillin and cloxacillin. The proposed method was applied to the determination of the two β-lactams in water samples of an urban river. The recoveries of these two β-lactams were found to be in the range 84.2-96.4%, demonstrating that PPy-PA6-NFsM has a high extraction capability for these two antibiotics. The relative standard deviations, ranging from 2.26% to 5.29% for intraday measurements and from 2.38% to 7.02% for inter-day determinations, were derived respectively.