A comparison of gene expression in mouse liver and kidney in obstructive cholestasis utilizing high-density oligonucleotide microarray technology

• Published in: World journal of gastroenterology : WJG Vol. 12; no. 16; pp. 2536 - 2548
• Main Authors: Denk, Gerald U, Cai, Shi-Ying, Chen, Wen-Sheng, Lin, Aiping, Soroka, Carol J, Boyer, James L
• Format: Journal Article
• Language: English
• Published: United States Department of Medicine Ⅱ-Groβhadern, Ludwig Maximilians University, München, Germany%Liver Center, Yale University School of Medicine, New Haven, Connecticut, United States%Liver Center, Yale University School of Medicine, New Haven, Connecticut, United States 28.04.2006; Liver Center, Yale University School of Medicine, New Haven, Connecticut, United States; Department of Gastroenterology, Southwest Hospital, Third Military Medical University, Chongqing 400038,China%W. M. Keck Biotechnology Resource Laboratory, Yale University School of Medicine, New Haven, Connecticut,United States; Baishideng Publishing Group Co., Limited
• Subjects: 25-Hydroxyvitamin D3 1-alpha-Hydroxylase - genetics; Animals; Antigens, CD - genetics; Antigens, Differentiation, Myelomonocytic - genetics; Basic Research; Blotting, Northern; Cholestasis - metabolism; Cytochrome P-450 Enzyme System - genetics; Cytochrome P450 Family 7; Fluorescent Antibody Technique, Indirect; Gene Expression Profiling; Kidney - metabolism; Liver - metabolism; Male; Mice; Mice, Inbred C57BL; Oligonucleotide Array Sequence Analysis; Steroid Hydroxylases - genetics; Microarray; Bile duct ligation; Liver; Cholestasis; Kidney
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v12.i16.2536

To assess the effects of obstructive cholestasis on a wider range of gene expression using microarray technology. Male C57BL/6J mice underwent common bile duct ligation (BDL) and were matched with pair-fed sham-operated controls. After 7 d, the animals were sacrificed and total RNA was isolated from livers and kidneys. Equal amounts of RNA from each tissue were pooled for each group and hybridized to Affymetrix GeneChip MG-U74Av2 containing a total of 12488 probe sets. Data analysis was performed using GeneSpring 6.0 software. Northern analysis and immunofluorescence were used for validation. In sham-operated and BDL mice, 44 and 50% of 12488 genes were expressed in livers, whereas 49 and 51% were expressed in kidneys, respectively. Seven days after BDL, 265 liver and 112 kidney genes with GeneOntology annotation were up-regulated and 113 liver and 36 kidney genes were down-regulated in comparison with sham-operated controls. Many genes were commonly regulated in both tissues and metabolism-related genes represented the largest functional group. Following BDL, microarray analysis reveals a broad range of gene alterations in both liver and kidney.