Stem Cell Conditioned Media Contains Important Growth Factors and Improves In Vitro Buffalo Embryo Production

• Published in: Animal biotechnology Vol. 27; no. 2; pp. 118 - 125
• Main Authors: Bhardwaj, Rahul, Ansari, Matin M., Parmar, Mehtab S., Chandra, Vikash, Sharma, G. Taru
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01.01.2016
• Subjects: Animals; Blastocyst - drug effects; Buffalo; Buffaloes; Culture Media, Conditioned - chemistry; Culture Media, Conditioned - pharmacology; Embryo Culture Techniques - methods; Female; Fertilization in Vitro - drug effects; growth factors; In vitro embryo culture; Intercellular Signaling Peptides and Proteins - metabolism; Male; stem cell conditioned media; Stem Cells - cytology; Stem Cells - metabolism; growth factors; In vitro embryo culture; Buffalo; stem cell conditioned media
• ISSN: 1532-2378; 1049-5398; 1532-2378
• DOI: 10.1080/10495398.2015.1118383

The present study was designed to investigate the effect of MSCs-conditioned media (CM) on quality buffalo embryo production in vitro . MSCs were harvested from Wharton’s jelly of 2-3 month old fetus and MSCs CM was collected. Immunocytochemistry and western blot assay revealed that MSCs secrete several important growth factors viz. FGF-2, IGF-1, LIF, TGF-β, and VEGF. Slaughterhouse derived culture grade cumulus oocyte complexes (COCs) were matured and fertilized in vitro . Presumptive zygotes were divided in four groups and cultured in vitro in respective media viz. group I (100% mSOF), Group II (100% Knockout Media DMEM+SR), Group III (50% CM + 50% mSOF), and group IV (100% CM). It was found that though the cleavage rate did not changed significantly (p < 0.05), but blastocyst rate was increased significantly (p < 0.05) in Group III and IV (24.24 ± 1.34 and 23.29 ± 1.25, respectively) compared to group I and II (16.04 ± 1.46 and 17.72 ± 0.94, respectively). Similarly, TCN was significantly (p < 0.05) higher in 50% CM and 100% CM replacement group (93.33 ± 1.91 and 92.13 ± 1.04, respectively) than the other two groups. It can be concluded from the study that MSCs secrete several important growth factors and MSCs-CM can be effectively used for enhancement of quality buffalo embryo production in vitro.