Leptospirosis in equine fetuses, stillborn foals, and placentas

• Published in: Veterinary pathology Vol. 30; no. 4; p. 362
• Main Authors: Poonacha, K.B, Donahue, J.M, Giles, R.C, Hong, C.B, Petrites-Murphy, M.B, Smith, B.J, Swerczek, T.W, Tramontin, R.R, Tuttle, P.A
• Format: Journal Article
• Language: English
• Published: United States 01.07.1993
• Subjects: Animals; Antibodies, Bacterial - blood; CABALLOS; CHEVAL; Female; Fetal Death - microbiology; Fetal Death - pathology; Fetal Death - veterinary; Fetal Diseases - microbiology; Fetal Diseases - pathology; Fetal Diseases - veterinary; Horse Diseases - pathology; HORSES; Kidney - pathology; LEPTOSPIRA; LEPTOSPIRA INTERROGANS; Leptospira interrogans - immunology; LEPTOSPIROSE; LEPTOSPIROSIS; Leptospirosis - microbiology; Leptospirosis - pathology; Leptospirosis - veterinary; Liver - pathology; Placenta Diseases - microbiology; Placenta Diseases - pathology; Placenta Diseases - veterinary; Pregnancy
• ISSN: 0300-9858; 1544-2217
• DOI: 10.1177/030098589303000405

Leptospirosis was diagnosed in 51 equine fetuses and 16 stillborn foals with gestational ages from 3 1/2 to 11 months. Diagnosis was based on one or more of the following: positive fetal antibody titer, positive fluorescent antibody test, demonstration of spirochetes in kidney and/or placental sections stained by the Warthin-Starry technique, high leptospiral titers in aborting mares, or isolation of Leptospira spp. from fetal organs. Gross lesions were observed in 80.3% of the fetuses, stillborn foals, and placentas. Gross placental lesions included nodular cystic allantoic masses, edema, areas of necrosis of the chorion, and necrotic mucoid exudate coating the chorion. The liver (23 cases) was enlarged, mottled, and pale to yellow. The kidneys (seven cases) were swollen and edematous with pale white radiating streaks in cortex and medulla. Microscopic lesions were observed in 96% of fetuses, stillborn foals, and placentas. Placental lesions consisted of thrombosis, vasculitis, mixed inflammatory cell infiltration of the stroma and villi, cystic adenomatous hyperplasia of allantoic epithelium, and villous necrosis and calcification. Fetal lesions included hepatocellular dissociation, mixed leukocytic infiltration of the portal triads, giant cell hepatopathy, suppurative and nonsuppurative nephritis, pulmonary hemorrhages, pneumonia, and myocarditis. Spirochetes were demonstrated with the Warthin-Starry stain in the allantochorion and/or kidney of 69 of the 71 cases. Using the direct fluorescent antibody technique, 56/60 cases tested positively for leptospires. Leptospires were isolated from fetal tissues in 20/42 cases. Sixteen of the isolates were identified by restriction enzyme analysis as Leptospira interrogans serogroup Pomona serovar kennewicki; case Nos. 36 and 41 were serovar grippotyphosa.