Inhibitors of nitrogen fixation in extracts from Clostridium pasteurianum

The gases H 2, CO, N 2O, and NO specifically inhibited the “nitrogenase” system in extracts from Clostridium pasteurianum. Concentrations of inhibitor effective against N 2 fixation did not substantially inhibit pyruvate metabolism. All the inhibitors acted competitively, although H 2 inhibition was...

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Published inBiochimica et biophysica acta Vol. 111; no. 1; pp. 1 - 10
Main Authors Lockshin, A., Burris, R.H.
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 15.11.1965
Subjects
Azides - pharmacology
Carbon Monoxide - pharmacology
Clostridium - drug effects
Clostridium - metabolism
Cyanides - pharmacology
Hydrogen - pharmacology
In Vitro Techniques
Nitric Oxide - pharmacology
Nitrogen Fixation
Nitrous Oxide - pharmacology
Pyruvates - pharmacology
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ISSN0304-4165
0006-3002
1872-8006
DOI10.1016/0304-4165(65)90466-6

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Summary:The gases H 2, CO, N 2O, and NO specifically inhibited the “nitrogenase” system in extracts from Clostridium pasteurianum. Concentrations of inhibitor effective against N 2 fixation did not substantially inhibit pyruvate metabolism. All the inhibitors acted competitively, although H 2 inhibition was uncompetitive at a higher than optimal phosphate concentration. Evacuation, following preincubation with the gaseous inhibitors, relieved all inhibitions. Cyanide and azide were weak inhibitors of N 2 fixation. These results are discussed with respect to the nature of the N 2-binding site and the mechanism of N 2 fixation.
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ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/0304-4165(65)90466-6