One binge-type cycle of alcohol plus ketamine exposure induces emotional-like disorders associated with oxidative damage in adolescent female rats

• Published in: Biomedicine & pharmacotherapy Vol. 162; p. 114641
• Main Authors: da Silveira, Cinthia Cristina Menezes, Cartágenes, Sabrina de Carvalho, Kobayashi, Natália Harumi Corrêa, Farias, Sarah Viana, de Souza-Junior, Fábio José Coelho, Fernandes, Luanna Melo Pereira, do Prado, Alejandro Ferraz, Aragão, Walessa Alana Bragança, Lima, Rafael Rodrigues, Ferreira, Wallax Augusto Silva, de Oliveira, Edivaldo Herculano Correa, Mello Júnior, Fernando Augusto Rodrigues, Burbano, Rommel Mario Rodríguez, Fontes-Júnior, Enéas Andrade, Maia, Cristiane do Socorro Ferraz
• Format: Journal Article
• Language: English
• Published: France Elsevier Masson SAS 01.06.2023; Elsevier
• Subjects: Adolescence; Alcoholism; Animals; Anxiety; Depression; Ethanol; Ethanol - pharmacology; Female; Ketamine; Ketamine - pharmacology; Oxidative Stress; Prefrontal Cortex; Rats; Oxidative stress; Adolescence; Depression; Anxiety; Ketamine; Ethanol
• ISSN: 0753-3322; 1950-6007
• DOI: 10.1016/j.biopha.2023.114641

Drug abuse is a global public health problem among adolescents, with alcohol often used in association with other psychotropic drugs, such as ketamine. Considering the scarcity of evidence, this study aimed to investigate emotional behavioral effects induced by ethanol plus ketamine co-abuse, as well as oxidative biochemistry, and neurotrophic mediator in the prefrontal cortex and hippocampus in the early withdrawal of adolescent female rats. Animals were divided into control, ethanol, ketamine, and ethanol plus ketamine groups. The protocol administration was performed for 3 consecutive days (binge-like pattern). Behavioral assays of open field, elevated plus maze, and forced swim test were performed. After that, the prefrontal cortex and hippocampus were collected to evaluate oxidative biochemistry (reactive oxygen species-ROS; Antioxidant capacity against peroxyl radicals-ACAP; and lipid peroxidation). We found that isolated or combined ethanol and ketamine exposure displayed anxiety- and depressive-like profile, in a non-synergistically manner during early withdrawal. However, oxidative damage was aggravated in the co-administered animals than in isolated exposed subjects. We concluded that ethanol plus ketamine co-abuse may intensify oxidative damage in the hippocampus and prefrontal cortex in the early withdrawal of adolescent female rats, which was not reflected in the emotional behavioral phenotype. The datasets used and/or analyzed during the current investigation are available upon reasonable request from the corresponding author. [Display omitted] •Acute ethanol plus ketamine exposure during adolescence displays anxiogenic and depressant profile in rats.•Ethanol plus ketamine exposure in adolescent rats triggers reactive oxygen species on hippocampus and prefrontal cortex.•Adolescent binge-like co-exposure synergically elicits oxidative damage.