Development of a Rapid and Accurate Identification Method for Citrobacter Species Isolated from Pork Products Using a Matrix-Assisted Laser-Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS)

• Published in: Journal of Microbiology and Biotechnology Vol. 25; no. 9; pp. 1537 - 1541
• Main Authors: Kwak, Hye-Lim, Han, Sun-Kyung, Park, Sunghoon, Park, Si Hong, Shim, Jae-Yong, Oh, Mihwa, Ricke, Steven C., Kim, Hae-Yeong
• Format: Journal Article
• Language: English
• Published: Korea (South) Korean Society for Microbiology and Biotechnology 01.09.2015; 한국미생물·생명공학회
• Subjects: Bacteriological Techniques; Bacteriological Techniques - methods; Citrobacter; Citrobacter - chemistry; Citrobacter - classification; Citrobacter - genetics; Citrobacter - isolation & purification; DNA, Bacterial; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal; DNA, Ribosomal - chemistry; DNA, Ribosomal - genetics; Red Meat; Red Meat - microbiology; RNA, Ribosomal, 16S; RNA, Ribosomal, 16S - genetics; Sensitivity and Specificity; Sequence Analysis, DNA; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Time Factors; 생물학; MALDI-TOF MS; pork products; identification; Citrobacter sp
• ISSN: 1017-7825; 1738-8872
• DOI: 10.4014/jmb.1503.03071

Previous detection methods for Citrobacter are considered time consuming and laborious. In this study, we have developed a rapid and accurate detection method for Citrobacter species in pork products, using matrix-assisted laser desorption ionization time-of-flight (MALDI-TOF) mass spectrometry (MS). A total of 35 Citrobacter strains were isolated from 30 pork products and identified by both MALDI-TOF MS and 16S rRNA gene sequencing approaches. All isolates were identified to the species level by the MALDI-TOF MS, while 16S rRNA gene sequencing results could not discriminate them clearly. These results confirmed that MALDI-TOF MS is a more accurate and rapid detection method for the identification of Citrobacter species.