HvGSK1.1 Controls Salt Tolerance and Yield through the Brassinosteroid Signaling Pathway in Barley

• Published in: International journal of molecular sciences Vol. 25; no. 2; p. 998
• Main Authors: Kloc, Yuliya, Dmochowska-Boguta, Marta, Żebrowska-Różańska, Paulina, Łaczmański, Łukasz, Nadolska-Orczyk, Anna, Orczyk, Wacław
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 13.01.2024
• Subjects: 24-epibrassinolide (EBL); Abiotic stress; Amino acids; Barley; bikinin; biomass; BR signaling pathway; brassinosteroids; Brassinosteroids - pharmacology; CRISPR; CRISPR/Cas9; Genes; Genome editing; Genomes; Glycogen Synthase Kinase 3 - genetics; Hordeum - genetics; Kinases; Mutation; Plant Growth Regulators; Plant resistance; Proteins; Salt; Salt Tolerance - genetics; Signal Transduction; BR signaling pathway; biomass; CRISPR/Cas9; brassinosteroids; bikinin; salt stress; kernel weight; 24-epibrassinolide (EBL)
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25020998

Brassinosteroids (BRs) are a class of plant steroid hormones that are essential for plant growth and development. BRs control important agronomic traits and responses to abiotic stresses. Through the signaling pathway, BRs control the expression of thousands of genes, resulting in a variety of biological responses. The key effectors of the BR pathway are two transcription factors (TFs): BRASSINAZOLE RESISTANT 1 (BZR1) and BRI1-EMSSUPPRESSOR 1 (BES1). Both TFs are phosphorylated and inactivated by the Glycogen synthase kinase 3 BRASSINOSTEROID INSENSITIVE2 (BIN2), which acts as a negative regulator of the BR pathway. In our study, we describe the functional characteristics of , which is one of the GSK3/SHAGGY-like orthologs in barley. We generated mutant lines of using CRISPR/Cas9 genome editing technology. Next Generation Sequencing (NGS) of the edited region of the showed a wide variety of mutations. Most of the changes (frameshift, premature stop codon, and translation termination) resulted in the knock-out of the target gene. The molecular and phenotypic characteristics of the mutant lines showed that the knock-out mutation of improved plant growth performance under salt stress conditions and increased the thousand kernel weight of the plants grown under normal conditions. The inactivation of HvGSK1.1 enhanced BR-dependent signaling, as indicated by the results of the leaf inclination assay in the edited lines. The plant traits under investigation are consistent with those known to be regulated by BRs. These results, together with studies of other gene members in other plant species, suggest that targeted editing of these genes may be useful in creating plants with improved agricultural traits.