FW2.2 and cell cycle control in developing tomato fruit: a possible example of gene co-option in the evolution of a novel organ

• Published in: Plant molecular biology Vol. 62; no. 6; pp. 867 - 880
• Main Authors: Cong, Bin, Tanksley, Steven D
• Format: Journal Article
• Language: English
• Published: Netherlands Springer Nature B.V 01.12.2006
• Subjects: Amino Acid Sequence; Arabidopsis - genetics; Arabidopsis - growth & development; Arabidopsis - metabolism; Biolistics - methods; Casein Kinase II - genetics; Casein Kinase II - metabolism; Cell cycle; Cell Cycle - genetics; Cell division; Cell Membrane - metabolism; Chromosome Mapping; DNA, Complementary - genetics; DNA, Complementary - metabolism; Evolution, Molecular; Fruit - genetics; Fruit - growth & development; Fruits; Gene Dosage; Gene Expression Regulation, Plant; Kinases; Luminescent Proteins - genetics; Luminescent Proteins - metabolism; Lycopersicon esculentum; Lycopersicon esculentum - genetics; Lycopersicon esculentum - growth & development; Microscopy, Confocal; Molecular Sequence Data; Mutation - genetics; Onions - cytology; Onions - metabolism; Phylogeny; Protein Binding; Proteins; Quantitative Trait Loci - genetics; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Saccharomyces cerevisiae - genetics; Sequence Homology, Amino Acid; Signal transduction; Tomatoes; Two-Hybrid System Techniques; Yeasts
• ISSN: 0167-4412; 1573-5028
• DOI: 10.1007/s11103-006-9062-6

fw2.2 is one of the few QTLs thus far isolated from plants and the first one known to control fruit size. While it has been established that FW2.2 is a regulator (either directly or indirectly) of cell division, FW2.2 does not share sequence homology to any protein of known function (Frary et al. Science 289:85-88, 2000; Cong et al. Proc Natl Acad Sci USA 99:13606-13611, 2002; Liu et al. Plant Physiol 132:292-299, 2003). Thus, the mechanism by which FW2.2 mediates cell division in developing fruit is currently unknown. In an effort to remedy this situation, a combination of yeast two-hybrid screens, in vitro binding assays and cell bombardment studies were performed. The results provide strong evidence that FW2.2 physically interacts at or near the plasma membrane with the regulatory (beta) subunit of a CKII kinase. CKII kinases are well-studied in both yeast and animals where they form part of cell cycle related signaling pathway. Thus while FW2.2 is a plant-specific protein and regulates cell division in a specialized plant organ (fruit), it appears to participate in a cell-cycle control signal transduction pathway that predates the divergence of single- and multi-cellular organisms. These results thus provide a glimpse into how ancient and conserved regulatory processes can be co-opted in the evolution of novel organs such as fruit.