Isolation and properties of the FSH and LH-releasing hormone

• Published in: Biochemical and biophysical research communications Vol. 43; no. 2; pp. 393 - 399
• Main Authors: Schally, A.V., Arimura, A., Baba, Y., Nair, R.M.G., Matsuo, H., Redding, T.W., Debeljuk, L., White, W.F.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 16.04.1971
• Subjects: Amino Acids - analysis; Animals; Castration; Chromatography, Gel; Countercurrent Distribution; Electrophoresis; Estrogens - pharmacology; Female; Follicle Stimulating Hormone; Freeze Drying; Hydrogen-Ion Concentration; Hydrolysis; Hypothalamus - analysis; Luteinizing Hormone - blood; Male; Methods; Ovary - physiology; Peptides; Pituitary Gland - drug effects; Pituitary Hormone-Releasing Hormones - analysis; Pituitary Hormone-Releasing Hormones - isolation & purification; Pituitary Hormone-Releasing Hormones - pharmacology; Progesterone - pharmacology; Radioimmunoassay; Rats; Spectrophotometry; Stimulation, Chemical; Swine; Testis - physiology; Testosterone - pharmacology
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/0006-291X(71)90766-2

LH-releasing hormone (LH-RH) was obtained in apparently a homogeneous state from extracts of pig hypothalami. The LH-RH preparation isolated has FSH-releasing hormone (FSH-RH) activity, which appears to be intrinsic to LH-RH. The amino acid composition of LH-RH/FSH-RH as determined on acid hydrolysates is: His 1, Arg 1, Ser 1, Glu 1, Pro 1, Gly 2, Leu 1 and Tyr 1. The hormone isolated stimulates the release of FSH and LH in vivo and in vitro in doses of a few nanograms. This polypeptide appears to represent the hypothalamic hormone which controls the secretion of both LH and FSH from the pituitary.