Characterization and Comparison of Steroidal Glycosides from Polygonatum Species by High-Performance Liquid Chromatography-Electrospray Ionization Mass Spectrometry

• Published in: Molecules (Basel, Switzerland) Vol. 28; no. 2; p. 705
• Main Authors: Liu, Danyang, Kikuchi, Takashi, Li, Wei
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 10.01.2023; MDPI
• Subjects: Chromatography; Chromatography, High Pressure Liquid - methods; Chromatography, Liquid; Electrospraying; Functional foods & nutraceuticals; Glycosides; Glycosides - chemistry; High performance liquid chromatography; HPLC-ESI-MS; Ionization; Ions; Liquid chromatography; Mass spectrometry; Mass spectroscopy; Phytochemicals; Polygonatum; Polygonatum - chemistry; Polygonatum species; Quality control; Scientific imaging; Spectrometry, Mass, Electrospray Ionization - methods; Spectroscopy; steroidal glycosides; Europe; Japan; Polygonatum species; HPLC-ESI-MS; steroidal glycosides
• ISSN: 1420-3049; 1420-3049
• DOI: 10.3390/molecules28020705

species have been used as traditional medicines and functional foods in Asia and Europe since ancient times. In this study, a fast and simple method based on liquid chromatography coupled with electrospray ionization mass spectrometry (LC-ESI-MS) was developed to systematically analyze and identify the steroidal glycosides in four major species distributed in Japan, including , , , and . As a result, 31 steroidal glycosides were tentatively identified, including 18 known and 13 previously unreported glycosides. Their structures were identified by the interpretation of chromatographic behavior and ESI-MS fragmentation patterns. The identification of 31 steroidal glycosides was indicative of a common biogenetic pathway in species. Our study disclosed the chemical profiling of steroidal glycosides in the plants of species, which will benefit better phytochemotaxonomical and phytochemical understanding and quality control for their medicinal usage.