Do Cuticular Gaps Make It Possible to Study the Composition of the Cell Walls in the Glands of Drosophyllum lusitanicum ?

• Published in: International journal of molecular sciences Vol. 25; no. 2; p. 1320
• Main Authors: Płachno, Bartosz J, Kapusta, Małgorzata, Stolarczyk, Piotr, Świątek, Piotr
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 21.01.2024
• Subjects: Animals; Antibodies; arabinogalactan proteins; carbohydrate epitopes; carnivorous plants; Caryophyllales; Cell Membrane; Cell Wall; Cells; cuticle; cuticular gaps; Flowers & plants; Leaves; Nutrients; Plant Leaves; Plants; VOCs; Volatile organic compounds; arabinogalactan proteins; Drosophyllaceae; carbohydrate epitopes; transfer cells; cuticle; mucilage glands; scanning transmission electron microscopy; cell wall; cuticular gaps; carnivorous plants
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms25021320

Carnivorous plants can survive in poor habitats because they have the ability to attract, capture, and digest prey and absorb animal nutrients using modified organs that are equipped with glands. These glands have terminal cells with permeable cuticles. Cuticular discontinuities allow both secretion and endocytosis. In , these emergences have glandular cells with cuticular discontinuities in the form of cuticular gaps. In this study, we determined whether these specific cuticular discontinuities were permeable enough to antibodies to show the occurrence of the cell wall polymers in the glands. Scanning transmission electron microscopy was used to show the structure of the cuticle. Fluorescence microscopy revealed the localization of the carbohydrate epitopes that are associated with the major cell wall polysaccharides and glycoproteins. We showed that leaf epidermal cells have a continuous and well-developed cuticle, which helps the plant inhibit water loss and live in a dry environment. The cuticular gaps only partially allow us to study the composition of cell walls in the glands of . We recoded arabinogalactan proteins, some homogalacturonans, and hemicelluloses. However, antibody penetration was only limited to the cell wall surface. The localization of the wall components in the cell wall ingrowths was missing. The use of enzymatic digestion improves the labeling of hemicelluloses in glands.