Sonodynamically induced anti-tumor effect with protoporphyrin IX on hepatoma-22 solid tumor

• Published in: Ultrasonics Vol. 51; no. 5; pp. 539 - 546
• Main Authors: Wang, Xiaobing, Wang, Yu, Wang, Pan, Cheng, Xiaoxia, Liu, Quanhong
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.07.2011; Elsevier
• Subjects: Animals; Anti-oxidative enzymes; Anti-tumor effect; Antineoplastic Agents - therapeutic use; Biocompatibility; Biological and medical sciences; Biomedical materials; Carcinoma, Hepatocellular - pathology; Carcinoma, Hepatocellular - therapy; Cell Line, Tumor; Enzymes; H-22 solid tumor; In vivo testing; In vivo tests; Liver Neoplasms - pathology; Liver Neoplasms - therapy; Medical sciences; Mice; Mice, Inbred ICR; Protoporphyrin IX; Protoporphyrins - therapeutic use; Radiation-Sensitizing Agents - therapeutic use; Radiotherapy. Instrumental treatment. Physiotherapy. Reeducation. Rehabilitation, orthophony, crenotherapy. Diet therapy and various other treatments (general aspects); Sonodynamic therapy; Surgical implants; Technology. Biomaterials. Equipments. Material. Instrumentation; Therapy; Treatment Outcome; Treatment. General aspects; Tumors; Ultrasonic Therapy - methods; H-22 solid tumor; Anti-tumor effect; Protoporphyrin IX; Sonodynamic therapy; Anti-oxidative enzymes; Therapy; Acoustic treatment; Rodentia; Ultrasonic treatment; Temperature effect; Hepatocellular carcinoma; Cytotoxicity; Synergism; Vertebrata; Mammalia; Transmission electron microscopy; Protoporphyrin; Mouse; Muscle; Skin; Biological effect; Pharmacokinetics; Damaging
• ISSN: 0041-624X; 1874-9968
• DOI: 10.1016/j.ultras.2010.12.001

► Protoporphyrin IX in H-22 tumors was relatively higher at 8–36 h after administration. ► Three times treatment inhibited 59.89% tumor growth and 45.86% tumor weight. ► The mice had a good vigor without so much weight change by SDT treatment. ► Obvious tissue destruction and decreased anti-oxidative enzymes were observed by SDT. ► An oxidative stress may be involved in PPIX-SDT in vivo. The purpose of this study was to evaluate sonodynamically induced anti-tumor effect of protoporphyrin IX (PPIX) in mice bearing hepatoma-22 (H-22) solid tumors, and the possible in vivo cell damage mechanism was also investigated. The pharmacokinetics of PPIX was analyzed in plasma, skin, muscle and tumor of H-22 bearing mice. Tumors were irradiated with ultrasound (1.43 MHz, I SATA 3 W/cm 2, 3 min) for three times at 8, 12 and 24 h after 5.0 mg/kg PPIX administration, respectively. The anti-tumor effects of sonodynamic therapy (SDT) were estimated by the tumor inhibition ratio (volume and weight). The bio-effects of SDT were evaluated by hematoxylin and eosin (H&E) staining, transmission electron microscope (TEM) observation, lipid peroxidation (LPO) measurement and anti-oxidative enzymes (glutathione peroxidase (GSH-PX), catalase (CAT) and superoxide dismutase (SOD)) assay. A significant anti-tumor effect was obtained by PPIX-mediated sonodynamic therapy (PPIX-SDT). At the fifteenth day after PPIX-SDT, the tumor growth and tumor weight inhibition ratios were 53.84% and 45.86%, respectively. In addition, the structure of tumor tissues and the anti-oxidative enzymes were obviously destroyed after SDT treatment. A biochemical mechanism was involved in PPIX-SDT in vivo, and the free radicals produced by the synergistic treatment destroying the anti-oxidative system of tumor cells in vivo may play an important role in this action. Also, the thermal effect could not be excluded in inducing damage of cellular structures, like membrane disruption and chromatin condensation under current evaluation in this paper.