2-bromoacetamide exposure impairs oocyte maturation in mice and humans primarily via disrupting the cytoskeleton

• Published in: Ecotoxicology and environmental safety Vol. 272; p. 116105
• Main Authors: Zhang, Chuanxin, Yin, Xiaoyu, Dong, Xueqi, Shi, Mingze, Xu, Yuxin, Gao, Jiayin, Wang, Jiawei, Song, Jinzhu, Liu, Boyang, Wu, Keliang
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Inc 01.03.2024; Elsevier
• Subjects: 2-bromoacetamide; Animals; Cytoskeleton; Cytoskeleton - metabolism; Humans; Mammals; Mice; Microtubules - metabolism; Mitochondria - metabolism; Oocyte maturation; Oocytes - metabolism; Oogenesis; Single-cell RNA sequencing; IVM; HAcAm; 2-bromoacetamide; GVBD; BAcAm; DEGs; PBE; scRNA-seq; Single-cell RNA sequencing; MI; GSH; RT-qPCR; Oocyte maturation; PBT; AI; GO; MII; ER; GV; PCA; MMP; ROS; IVF; Cytoskeleton; DBPs
• ISSN: 0147-6513; 1090-2414
• DOI: 10.1016/j.ecoenv.2024.116105

2-bromoacetamide (BAcAm) is an emerging class of unregulated disinfection by-products (DBPs), with potent cytogenetic and developmental toxicity in animals. However, whether BAcAm exerts toxic effects on mammalian oocyte quality remains to be elucidate. In this research, we investigated the effect of BAcAm on mouse and human oocyte maturation with an in vitro culture system. Our results revealed that BAcAm exposure hindered the extrusion of the first polar body, disrupted the spindle organization and reduced the competence of embryo development after fertilization in the mouse oocytes. Results of single-cell RNA sequencing (scRNA-seq) showed that 605 differentially expressed genes (DEGs) were identified in the BAcAm exposed mouse oocytes, among which 366 were up-regulated and 239 were down-regulated. Gene Ontology (GO) analysis further revealed that DEGs were mainly enriched in mitochondrial functions, oxidative stress, cytoskeleton, endoplasmic reticulum (ER), Golgi and protein synthesis, DNA damage and apoptosis. We then conducted further tests in these aspects and discovered that BAcAm exposure principally perturbed the function of microtubule and actin cytoskeleton. This finding was confirmed in human oocytes. Overall, our data suggest that BAcAm exposure disturbs the cytoskeleton function, thus impairing oocyte maturation. These data, for the first time, provide a comprehensive view for the toxic effects of BAcAm on oocyte maturation. [Display omitted] •BAcAm exposure hindered oocyte meiotic maturation and decreased oocyte quality.•BAcAm exposure led to extensive alterations in gene expression.•BAcAm exposure disrupted microtubule and actin cytoskeleton.