The bacterial oxidation of indole

A microorganism was isolated from soil with indole as the sole source of carbon and nitrogen. Evidence was presented that indole was oxidized to anthranilic acid in this bacterium with dihydroxyindole as the intermediate. The enzyme catalyzing the oxidation of dihydroxyindole, designated as dihydrox...

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Bibliographic Details
Published inBiochimica et biophysica acta Vol. 158; no. 1; pp. 70 - 78
Main Authors Fujioka, Motoji, Wada, Hiroshi
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 16.04.1968
Subjects
Bacteria - enzymology
Bacteria - isolation & purification
Bacteria - metabolism
Indoles - metabolism
ortho-Aminobenzoates - metabolism
Oxidoreductases
Skatole - metabolism
Soil Microbiology
Spectrophotometry
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Summary:A microorganism was isolated from soil with indole as the sole source of carbon and nitrogen. Evidence was presented that indole was oxidized to anthranilic acid in this bacterium with dihydroxyindole as the intermediate. The enzyme catalyzing the oxidation of dihydroxyindole, designated as dihydroxyindole oxygenase, was purified about 65-fold from the sonic extract of the bacterium and some of the properties were investigated. The indole-grown cell was shown to oxidize skatole to (+)-2-oxo-3-methyl-3-hydroxyindoline. In connection with this finding, the of oxidation of indole to dihydroxyindole is discussed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
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ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/0304-4165(68)90073-1