Use of [ 3H]raffinose as a specific probe of autophagic sequestration

The trisaccharide [ 3H]raffinose, introduced into the cytosol of isolated rat hepatocytes by means of electropermeabilization, was sequestered autophagically and accumulated in lysosomes and pre-lysosomal vacuoles. In contrast to the disaccharide [ 14C]sucrose previously used as a sequestration prob...

Full description

Saved in:
Bibliographic Details
Published inExperimental cell research Vol. 162; no. 1; pp. 273 - 277
Main Authors Seglen, Per O., Gordon, Paul B., Tolleshaug, Helge, Høyvik, Henrik
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 1986
Subjects
Adenine - analogs & derivatives
Adenine - pharmacology
Animals
Autophagy - drug effects
Digitonin
Liver - drug effects
Liver - metabolism
Liver - ultrastructure
Lysosomes - metabolism
Male
Mitochondria, Liver - metabolism
Oligosaccharides - metabolism
Phagocytosis - drug effects
Phagosomes - metabolism
Raffinose - metabolism
Rats
Rats, Inbred Strains
Sucrose - metabolism
Online AccessGet full text

Cover

More Information
Summary:The trisaccharide [ 3H]raffinose, introduced into the cytosol of isolated rat hepatocytes by means of electropermeabilization, was sequestered autophagically and accumulated in lysosomes and pre-lysosomal vacuoles. In contrast to the disaccharide [ 14C]sucrose previously used as a sequestration probe, raffinose was not taken up by the mitochondria. The sequestration of raffinose was completely inhibited by the autophagy suppressor 3-methyladenine, stressing its potential utility as a specific probe of hepatocytic autophagy.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0014-4827
1090-2422
DOI:10.1016/0014-4827(86)90446-5