Biomonitoring of human genotoxicity induced by complex occupational exposures

• Published in: Mutation research Vol. 445; no. 2; pp. 193 - 203
• Main Authors: Schoket, Bernadette, Poirier, Miriam C, Mayer, Gábor, Török, Géza, Kolozsi-Ringelhann, Ágnes, Bognár, Gabriella, Bigbee, William L, Vincze, István
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 30.09.1999
• Subjects: 1-Hydroxypyrene; [formula omitted]-postlabelling; Air Pollutants, Occupational - analysis; Aluminum; Aromatic DNA adduct; Biomarker; Biomarkers - blood; Biomarkers - urine; Chemical Industry; Competitive immunoassay; DNA Adducts - blood; DNA Damage; Environmental Monitoring; Female; Glycophorin - genetics; Glycophorin - metabolism; Glycophorin A; Human biomonitoring; Humans; Imidazoles; Longitudinal Studies; Lymphocytes - drug effects; Male; Metallurgy; Micronucleus Tests; Occupational exposure; Occupational Exposure - adverse effects; Occupational Exposure - analysis; Phosphorus Radioisotopes; Polycyclic aromatic hydrocarbon; Pyrenes - analysis; Rubber; Sensitivity and Specificity; Vehicle Emissions - adverse effects; Vehicle Emissions - analysis; Glycophorin A; Biomarker; SCE, sister chromatid exchange; G-C8-4-ABP, guanosin-(8-yl)-4-aminobiphenyl; BPdG, (7 R)- N 2-{10-[7β,8α,9α-trihydroxy-7,8,9,10-tetrahydrobenzo( a)pyrene]-yl}-deoxyguanosine; Polycyclic aromatic hydrocarbon; PAH, polycyclic aromatic hydrocarbon; DELFIA, dissociation-enhanced lanthanide fluoroimmunoassay; Occupational exposure; ELISA, enzyme-linked immunosorbent assay; G-C8-AAF, guanosin-(8-yl)-acetylaminofluorene; GPA, glycophorin A; 1-Hydroxypyrene; 32 P -postlabelling; 1-OH-PY, 1-hydroxypyrene; Aromatic DNA adduct; Human biomonitoring; Competitive immunoassay
• ISSN: 1383-5718; 0027-5107; 1879-3592
• DOI: 10.1016/S1383-5718(99)00126-6

Sensitivity, specificity and correlations among several biomarkers for monitoring occupational exposure to complex mixtures of genotoxic agents were assessed in occupational environments in Hungarian study populations. The studies have been focused on DNA adduct formation, urinary metabolites, mutations and micronuclei induced by exposures to complex organic mixtures. In two Hungarian aluminium plants, increased DNA adduct and 1-hydroxypyrene (1-OH-PY) levels were observed in workers as compared to controls. However, no association between the biomarker levels was evident on an individual basis. In Hungarian garage mechanics, DNA adduct determinations did not show increased genotoxic exposure as compared to the controls. However, ambient air measurements, significantly enhanced 1-OH-PY levels, and slightly enhanced frequency of micronuclei indicated increased polycyclic aromatic hydrocarbon (PAH) exposure in the garages, as compared to the general environment. In a Hungarian vulcanizing plant, DNA adduct determinations and 1-OH-PY did not show significantly elevated exposure levels as compared to controls. The glycophorin A (GPA) somatic mutation assay was also negative for this occupational exposure. The results support previous observations of a lack of correlation between DNA adducts detectable by 32 P -postlabelling and those measured by the PAH–DNA immunoassay in the same DNA sample. These studies also demonstrate a lack of close correlation between levels of DNA adducts and urinary 1-OH-PY in the same individual.