Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains

• Published in: Annals of Agricultural and Environmental Medicine Vol. 26; no. 1; pp. 198 - 202
• Main Authors: Padzik, Marcin, Hendiger, Edyta B, Żochowska, Agnieszka, Szczepaniak, Jarosław, Baltaza, Wanda, Pietruczuk-Padzik, Anna, Olędzka, Gabriela, Chomicz, Lidia
• Format: Journal Article
• Language: English
• Published: Poland Institute of Rural Health 22.03.2019
• Subjects: Acanthamoeba; Acanthamoeba castellanii - drug effects; Acanthamoeba keratitis; Acanthamoeba Keratitis - prevention & control; Acanthamoeba trophozoites; Amoeba; anti-amoebic in vitro effect; Cell Line, Tumor; contact lens solutions; Contact Lens Solutions - pharmacology; Contact Lens Solutions - toxicity; Contact lenses; Cytotoxicity; Disinfection; Eye diseases; Gold - pharmacology; Gold - toxicity; Growth inhibition; Health risks; Humans; Hygiene; Infectious diseases; Introduced species; Keratitis; L-Lactate dehydrogenase; L-Lactate Dehydrogenase - metabolism; Lactic acid; Metal Nanoparticles - therapeutic use; Metal Nanoparticles - toxicity; Nanoparticles; Nanotechnology; Parasites; Prevention; Protozoa; Public health; Silver; Silver - pharmacology; Silver - toxicity; silver/gold nanoparticles; Trophozoites; Trophozoites - drug effects; Acanthamoeba trophozoites; Acanthamoeba keratitis; contact lens solutions; anti-amoebic in vitro effect; silver/gold nanoparticles
• ISSN: 1232-1966; 1898-2263
• DOI: 10.26444/aaem/105394

Various species are free-living organisms widely distributed in the human environment. Amphizoic amoebae as facultative parasites may cause vision-threatening eye disease - keratitis, mostly among contact lens wearers. As the number of cases is increasing, and applied therapy often unsuccessful, proper hygienic measures and effective contact lenses disinfection are crucial for the prevention of this disease. Available contact lens solutions are not fully effective against amphizoic amoebae; there is a need to enhance their disinfecting activity to prevent amoebic infections. The use of developing nanotechnology methods already applied with success in the prevention, diagnostic and therapy of other infectious diseases might be helpful regarding amoebic keratitis. This study assesses the in vitro effect of selected contact lens solutions conjugated with nanoparticles against trophozoites. Three selected contact lens solutions conjugated with silver and gold nanoparticles in concentration of 0.25-2.5 ppm were used in vitro against the axenically cultured ATCC 30010 type castellanii strain. The anti-amoebic efficacy was examined based on the oxido-reduction of AlamarBlue. The cytotoxicity tests based on the measurement of lactate dehydrogenase (LDH) activity were performed using a fibroblast HS-5 cell line. Enhancement of the anti-amoebic activity of contact lens solutions conjugated with selected nanoparticles expressed in the dose dependent amoebic growth inhibition with a low cytotoxicity profile was observed. Results of the study showed that conjugation of selected contact lens solutions with silver nanoparticles might be a promising approach to prevent keratitis among contact lens users.