Separation and quantitation of saccharides by ion-exchange chromatography utilizing boric acid/glycerol buffers

Two chromatographic systems for the separation and quantitation of saccharides have been described. These separations are based on the ion-exchange of the sugar-borate complexes, elution being performed using boric acid/glycerol buffers at pH 6.7 and elevated operating temperatures. The use of a low...

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Published inAnalytical biochemistry Vol. 22; no. 1; pp. 123 - 133
Main Authors Walborg, Earl F., Lantz, Roberta S.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 1968
Subjects
Arabinose - analysis
Boric Acids
Chromatography, Ion Exchange
Disaccharides - analysis
Fructose - analysis
Fucose - analysis
Galactose - analysis
Glucose - analysis
Glycerol
Hexoses - analysis
Lactose - analysis
Maltose - analysis
Mannose - analysis
Monosaccharides - analysis
Pentoses - analysis
Polysaccharides - analysis
Rhamnose - analysis
Ribose - analysis
Sucrose - analysis
Xylose - analysis
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Summary:Two chromatographic systems for the separation and quantitation of saccharides have been described. These separations are based on the ion-exchange of the sugar-borate complexes, elution being performed using boric acid/glycerol buffers at pH 6.7 and elevated operating temperatures. The use of a lower cross-linked resin, Dowex 1-X4, has permitted the chromatographic separations to be performed at increased flow rates and has demonstrated the applicability of this technique to the separation of di- and trisaccharides. Analysis of the column effluents utilizing the aniline/acetic, orthophosphoric acid reagent permits quantitation of the saccharides with a precision of ±5%.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0003-2697
1096-0309
DOI:10.1016/0003-2697(68)90266-2