Separation and quantitation of saccharides by ion-exchange chromatography utilizing boric acid/glycerol buffers
Two chromatographic systems for the separation and quantitation of saccharides have been described. These separations are based on the ion-exchange of the sugar-borate complexes, elution being performed using boric acid/glycerol buffers at pH 6.7 and elevated operating temperatures. The use of a low...
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Published in | Analytical biochemistry Vol. 22; no. 1; pp. 123 - 133 |
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Main Authors | , |
Format | Journal Article |
Language | English |
Published |
United States
Elsevier Inc
1968
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Subjects | |
Online Access | Get full text |
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Summary: | Two chromatographic systems for the separation and quantitation of saccharides have been described. These separations are based on the ion-exchange of the sugar-borate complexes, elution being performed using boric acid/glycerol buffers at pH 6.7 and elevated operating temperatures. The use of a lower cross-linked resin, Dowex 1-X4, has permitted the chromatographic separations to be performed at increased flow rates and has demonstrated the applicability of this technique to the separation of di- and trisaccharides. Analysis of the column effluents utilizing the aniline/acetic, orthophosphoric acid reagent permits quantitation of the saccharides with a precision of ±5%. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0003-2697 1096-0309 |
DOI: | 10.1016/0003-2697(68)90266-2 |