Metalloproteinases and their inhibitors: Influence on tumor invasiveness and metastasis formation in head and neck squamous cell carcinomas

• Published in: Head & neck Vol. 28; no. 1; pp. 31 - 39
• Main Authors: Görögh, Tibor, Beier, Ulf H., Bäumken, Jens, Meyer, Jens E., Hoffmann, Markus, Gottschlich, Stefan, Maune, Steffen
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.01.2006; John Wiley & Sons
• Subjects: Biological and medical sciences; Biopsy; Carcinoma, Squamous Cell - enzymology; Carcinoma, Squamous Cell - pathology; Cell Line; DNA Primers; Electrophoresis, Polyacrylamide Gel; Enzymes; Female; Gel electrophoresis; Gelatinase A; Gelatinase B; gene expression; Gene Expression Regulation, Enzymologic; Gene Expression Regulation, Neoplastic; Head; Head and neck cancer; Head and Neck Neoplasms - enzymology; Head and Neck Neoplasms - pathology; HNSCC; Humans; Immunohistochemistry; Interstitial collagenase; Invasiveness; Lymph nodes; Male; Matrix metalloproteinase; Matrix Metalloproteinase Inhibitors; Matrix Metalloproteinases - metabolism; Medical sciences; Metastases; Middle Aged; MMP; Neck; Neoplasm Invasiveness; Otorhinolaryngology (head neck, general aspects and miscellaneous); Otorhinolaryngology. Stomatology; Polymerase Chain Reaction; Sequence Analysis; Silver; squamous cell carcinoma; Stromelysin 2; TIMP; Tissue inhibitor of metalloproteinase 1; Tissue inhibitor of metalloproteinase 2; Tissue Inhibitor of Metalloproteinase-1 - genetics; Tissue Inhibitor of Metalloproteinase-1 - metabolism; Tissue Inhibitor of Metalloproteinase-2 - genetics; Tissue Inhibitor of Metalloproteinase-2 - metabolism; Tissue inhibitor of metalloproteinases; Translation; Tumor Cells, Cultured; Tumors; Human; Enzyme; Pathogenesis; Stomatology; Enzyme inhibitor; Metalloendopeptidases; Tumorous infiltration; Malignant tumor; Metastasis; Gene expression; Peptidases; MMP; invasiveness; HNSCC; Hydrolases; ENT disease; Head and neck squamous cell carcinoma; TIMP
• ISSN: 1043-3074; 1097-0347
• DOI: 10.1002/hed.20298

Background. Matrix metalloproteinases (MMPs) play an important role in tumor invasiveness. This study investigates the expression status of MMPs and tissue inhibitors of metalloproteinases (TIMPs) in head and neck squamous cell carcinomas (HNSCC). Methods. Of 48 laryngeal squamous cell carcinoma (LSCC) biopsies and 10 HNSCC cell lines, mRNA was isolated, reversely transcribed, and subjected to polymerase chain reaction (PCR) amplifying MMP‐1, MMP‐2, MMP‐9, MMP‐10, TIMP‐1, and TIMP‐2. Silver nitrate–stained gel electrophoresis demonstrated MMP and TIMP expression status. Exemplary immunohistochemistry and zymography confirmed translation and enzyme activity. Results. Densitometric analysis revealed MMP‐2 expression and lymph node metastases to be positively and TIMP‐1 and TIMP‐2 to be negatively correlated with lymph node metastases. TIMP‐2 expression and tumor size were negatively correlated. MMP‐1, MMP‐9, and MMP‐10 expression were not correlated to metastasis formation or tumor size. Conclusions. Our results suggest that MMP‐2 expression enhances, whereas TIMP‐1 and TIMP‐2 both suppress, cancer spread in LSCC. © 2005 Wiley Periodicals, Inc. Head Neck 27: XXX–XXX, 2005