Molecular characterization of the mutable flaked allele for flower variegation in the common morning glory

• Published in: The Plant journal : for cell and molecular biology Vol. 16; no. 3; pp. 371 - 376
• Main Authors: Habu, Yoshiki, Hisatomi, Yasuyo, Iida, Shigeru
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.11.1998; Blackwell Science
• Subjects: Agronomy. Soil science and plant productions; Alleles; Base Sequence; Biological and medical sciences; DNA Primers; DNA Transposable Elements; Fundamental and applied biological sciences. Psychology; Genetics and breeding of economic plants; Ipomoea; Metabolism; Molecular Sequence Data; Mutation; Pigmentation - genetics; Plant physiology and development; Plants - genetics; Storage and secretion, pigments, phytochrome; Varietal selection. Specialized plant breeding, plant breeding aims; Yield, quality, earliness, varia; Genetic mapping; Flower; Transposable element; Tandemly repeated sequence; Convolvulaceae; Genetic determinism; Phenotype; Ipomoea; Variegation; Gene; Dicotyledones; Insertion mutation; Angiospermae; Flower crop; Spermatophyta; Sequencing; Coloration
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1046/j.1365-313x.1998.00308.x

Summary The mutable flaked (or af ) lines of the common morning glory bear white flowers with colored flakes and sectors. The af allele shows incomplete dominance. Plants in the heterozygous state A/af bear lightly colored flowers with intensely colored flakes and occasionally with white sectors. We showed that the mutable af allele is caused by insertion of a new transposable element, Tip100 , into the CHS‐D gene intron. Tip100 is 3.9 kb long and belongs to the Ac/Ds family. Although the timing and frequency of the flower variegation vary in different lines, they carry an identical mutable allele. We also noticed that a flaked subline, bearing variegated flowers, carries a Tip100 derivative, Tip100–1 . The structure of Tip100–1 contains an additional 48 bp terminal sequence as tandem repeats and its integration site is identical to that of Tip100 . Another line, with stable white flowers, is a double mutant carrying two copies of Tip100 in the CHS‐D gene. These results are discussed with regard to the variegated phenotypes of flowers in various mutable lines.