High hydrostatic pressure extract of Siegesbeckia orientalis inhibits adipogenesis through the activation of the Wnt/β-catenin signaling pathway

• Published in: Food science and biotechnology Vol. 29; no. 7; pp. 977 - 985
• Main Authors: Kim, Mi-Bo, Kim, Changhee, Hwang, Jae-Kwan
• Format: Journal Article
• Language: English
• Published: Singapore Springer Singapore 01.07.2020; Springer Nature B.V; 한국식품과학회
• Subjects: Acetyl-CoA carboxylase; Adipocytes; Adipogenesis; Adiponectin; CCAAT/enhancer-binding protein; Chemistry; Chemistry and Materials Science; Fatty acids; Fatty-acid synthase; Food Science; Gene expression; Hydrostatic pressure; Infertility; Inflammation; Kinases; Leptin; Lipids; Nutrition; Proteins; Siegesbeckia orientalis; Signal transduction; Signaling; siRNA; Sterol regulatory element-binding protein; Wnt protein; Wort; β-Catenin; 식품과학; Wnt/β-catenin system; High hydrostatic pressure; St. Paul’s Wort; Adipogenesis
• ISSN: 1226-7708; 2092-6456
• DOI: 10.1007/s10068-020-00733-7

St. Paul’s Wort ( Siegesbeckia orientalis L.) confers anti-oxidative, anti-inflammatory, anti-allergic, anti-infertility, and immunosuppressive properties. Here, we elucidated whether high hydrostatic pressure extract of St. Paul’s Wort (SPW-HHPE) had anti-adipogenic activity. SPW-HHPE inhibited adipogenesis by reducing intracellular lipid accumulation. SPW-HHPE reduced the mRNA and protein expression of adipogenic regulatory factors [peroxisome proliferator-activated receptor gamma (PPARγ), CCAAT/enhancer binding protein alpha (C/EBPα), and sterol regulatory element binding protein-1c]. In addition, SPW-HHPE decreased the mRNA expression levels of lipogenic enzymes (fatty acid synthase and acetyl-CoA carboxylase) as well as adipocytokines (adiponectin and leptin). The inhibitory effect of SPW-HHPE on adipogenesis was mainly attributed to the enhancement of the Wnt/β-catenin signaling pathway. When β-catenin siRNA was transfected into 3T3-L1 adipocytes, the mRNA expression of PPARγ and C/EBPα was upregulated; however, their expression was attenuated by SPW-HHPE. These results suggest that SPW-HHPE suppresses adipogenesis by stimulating Wnt/β-catenin pathway.