Expression of cyclic nucleotide-gated channels in the rat medial vestibular nucleus

The role of cyclic nucleotide-gated (CNG) channels in sensory signal transduction in retinal and olfactory cells is widely recognized, but there is increasing evidence that they also play more general functions in the central nervous system as downstream effectors of cyclic nucleotides. Here, we dem...

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Bibliographic Details
Published inNeuroreport Vol. 16; no. 17; p. 1939
Main Authors Podda, Maria V, Marcocci, Maria E, Del Carlo, Barbara, Palamara, Anna T, Azzena, Gian Battista, Grassi, Claudio
Format Journal Article
LanguageEnglish
Published England 28.11.2005
Subjects
Animals
Cyclic Nucleotide-Gated Cation Channels
Gene Expression
Ion Channels - genetics
Ion Channels - metabolism
Male
Organ Culture Techniques
Polymerase Chain Reaction
Rats
Rats, Wistar
RNA, Messenger - analysis
Vestibular Nuclei - metabolism
Vestibular Nuclei - physiology
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Summary:The role of cyclic nucleotide-gated (CNG) channels in sensory signal transduction in retinal and olfactory cells is widely recognized, but there is increasing evidence that they also play more general functions in the central nervous system as downstream effectors of cyclic nucleotides. Here, we demonstrate the expression of the alpha-subunit of rod- and olfactory-type CNG channels (CNG1 and CNG2, respectively) in the rat medial vestibular nucleus (MVN). Nested polymerase chain reaction revealed CNG channel mRNA in the MVN, and CNG1 and CNG2 proteins were also detected by Western blotting and immunohistochemistry. Finally, electrophysiological evidence is provided suggesting that CNG channels play a functional role in the MVN.
ISSN:0959-4965
DOI:10.1097/01.wnr.0000187633.91375.c8