Systematic Analysis of Steroids. IV. Thin-layer Chromatography and Densitometry of Bile Components

Bile from various animals was submitted directly to thin-layer chromatography, and free and conjugated bile acids and cholesterol in the bile were separated. The chromatogram was analyzed by densitometer with autorecording calculator and animal species difference was revealed by the densitogram. Den...

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Bibliographic Details
Published inChemical & pharmaceutical bulletin Vol. 12; no. 4; pp. 483 - 488
Main Authors Hara, Shoji, Takeuchi, Michiko, Tachibana, Misako, Chihara, Goro
Format Journal Article
LanguageEnglish
Published Japan The Pharmaceutical Society of Japan 1964
Japan Science and Technology Agency
Subjects
Bile
Bile Acids and Salts
Cholesterol
Chromatography
Chromatography, Thin Layer
Densitometry
Old Medline
Salts
Steroids
CHOLESTEROL
CHROMATOGRAPHY
BILE ACIDS AND SALTS
EXPERIMENTAL LAB STUDY
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Summary:Bile from various animals was submitted directly to thin-layer chromatography, and free and conjugated bile acids and cholesterol in the bile were separated. The chromatogram was analyzed by densitometer with autorecording calculator and animal species difference was revealed by the densitogram. Densitometric analysis gives semiquantitative results and this can be used for clinical chemical analysis.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0009-2363
1347-5223
DOI:10.1248/cpb.12.483