Dissection of multigenic obesity traits in congenic mouse strains

Previous quantitative trait locus mapping (QTL) identified multigenic obesity (MOB) loci on mouse Chromosome (Chr) 2 that influence the interrelated phenotypes of obesity, insulin resistance, and dyslipidemia. To better localize and characterize the MOB locus, three congenic mouse strains were creat...

Full description

Saved in:
Bibliographic Details
Published inMammalian genome Vol. 15; no. 1; pp. 14 - 22
Main Authors Estrada-Smith, Daria, Castellani, Lawrence W, Wong, Howard, Wen, Ping-Zi, Chui, Aileen, Lusis, Aldons J, Davis, Richard C
Format Journal Article
LanguageEnglish
Published United States Springer Nature B.V 01.01.2004
Subjects
Animals
Arteriosclerosis - genetics
Cholesterol - metabolism
Chromosome Mapping
Chromosomes - genetics
Crosses, Genetic
dyslipidemia
Genetic Markers
Insulin Resistance - genetics
Lipoproteins - metabolism
Male
metabolic syndrome
Mice
Mice, Congenic - genetics
Mice, Inbred C57BL
Obesity - genetics
Quantitative Trait, Heritable
Online AccessGet full text

Cover

More Information
Summary:Previous quantitative trait locus mapping (QTL) identified multigenic obesity (MOB) loci on mouse Chromosome (Chr) 2 that influence the interrelated phenotypes of obesity, insulin resistance, and dyslipidemia. To better localize and characterize the MOB locus, three congenic mouse strains were created. Overlapping genomic intervals from the lean CAST/Ei (CAST) strain were introgressed onto an obesity-susceptible C57BL/6 (BL6) background to create proximal (15 Mb-73 Mb), middle (63 Mb-165 Mb), and distal (83 Mb-182 Mb) congenic strains. The congenic strains showed differences in obesity, insulin, and lipid traits consistent with the original QTL analysis for the locus. Importantly, characterization of the MOB congenics localized the effects of genes that underlie obesity-related traits to an introgressed interval (73-83 Mb) unique to the middle MOB congenic. Conversely, significant differences between the lipid and insulin profiles of the middle and distal MOB congenics implicated the presence of at least two genes that underlie these traits. When fed an atherogenic diet, several traits associated with metabolic syndrome were observed in the distal MOB congenic, while alterations in plasma lipoproteins were observed in the middle MOB congenic strain.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ObjectType-Article-1
ObjectType-Feature-2
ISSN:0938-8990
1432-1777
DOI:10.1007/s00335-003-2294-8