Effects of targeting SLC1A5 on inhibiting gastric cancer growth and tumor development in vitro and in vivo

• Published in: Oncotarget Vol. 8; no. 44; pp. 76458 - 76467
• Main Authors: Lu, Jian, Chen, Min, Tao, Zhenhua, Gao, Sumeng, Li, Yang, Cao, Yu, Lu, Chun, Zou, Xiaoping
• Format: Journal Article
• Language: English
• Published: United States Impact Journals LLC 29.09.2017
• Subjects: Research Paper; cell motility; SLC1A5; cell proliferation; gastric cancer
• ISSN: 1949-2553; 1949-2553
• DOI: 10.18632/oncotarget.19479

To investigate the oncogenic effects of SLC1A5 on gastric cancer development and . The expression level of SLC1A5 was detected in 70 gastric cancer paraffin-embedded tissues by immunohistochemistry and also was detected in gastric cancer cell lines by qRT-PCR and western blotting analysis. The effects of knockdown SLC1A5 were analyzed on cell proliferation, cell cycle, the ability of cell migration and invasion and growth signaling pathway . By using subcutaneous xenograft mouse, the importance of SLC1A5 expression was assessed for both successful engraftment and growth of gastric cancer cells . SLC1A5 was up-regulated in gastric cancer tissues and was correlated with malignant features such as deeper local invasion, higher lymph node metastasis, advanced TNM stages and higher Ki-67 expression. Knockdown SLC1A5 in gastric cancer cells suppressed cell proliferation, caused G0/G1 arrest and inhibited cell invasion as well as migration partly by inactivated mTOR/p-70S6K1 signaling pathway . Furthermore, experiments indicated that suppression of SLC1A5 could inhibit relative volume of xenografted tumor. Our results suggested that SLC1A5 might be considered as a new biomarker and also as a potential therapeutic target in gastric cancer.