A gene controlling the synthesis of non specific alkaline phosphatase in Saccharomyces cerevisiae

Recessive mutants defective in the formation of non-specific alkaline phosphatase (EC 3.1.3.1) could be selected by staining colonies on a plate with p-nitrophenylphosphate after treatment with chloroform vapour. Since no complementation was observed among the nine mutants so far tested, all the mut...

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Bibliographic Details
Published inBiochimica et biophysica acta Vol. 428; no. 1; pp. 182 - 192
Main Authors Toh-e, Akio, Nakamura, Hideo, Oshima, Yasuji
Format Journal Article
LanguageEnglish
Published Netherlands Elsevier B.V 25.03.1976
Subjects
Alkaline Phosphatase - biosynthesis
Alkaline Phosphatase - isolation & purification
Alkaline Phosphatase - metabolism
Chromatography, Gel
Crosses, Genetic
Enzyme Repression
Genes, Regulator
Genotype
Mutation
Saccharomyces cerevisiae - enzymology
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Summary:Recessive mutants defective in the formation of non-specific alkaline phosphatase (EC 3.1.3.1) could be selected by staining colonies on a plate with p-nitrophenylphosphate after treatment with chloroform vapour. Since no complementation was observed among the nine mutants so far tested, all the mutations might occur in the same locus, phoH. The non-specific alkaline phosphatase was repressible, although a significant basal level of the enzyme activity was observed in the repressed condition.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0304-4165
0006-3002
1872-8006
DOI:10.1016/0304-4165(76)90119-7