Risk modification of colorectal cancer susceptibility by interleukin-8-251T〉A polymorphism in Malaysians

• Published in: World journal of gastroenterology : WJG Vol. 18; no. 21; pp. 2668 - 2673
• Main Authors: Mustapha, Mohd Aminudin, Shahpudin, Siti Nurfatimah Mohd, Aziz, Ahmad Aizat Abdul, Ankathil, Ravindran
• Format: Journal Article
• Language: English
• Published: United States Baishideng Publishing Group Co., Limited 07.06.2012
• Subjects: Adult; Aged; Alleles; Brief; Case-Control Studies; Colorectal Neoplasms - diagnosis; Colorectal Neoplasms - ethnology; Colorectal Neoplasms - genetics; Female; Fisher精确检验; Gene Frequency; Genetic Predisposition to Disease; Genetic Variation; Genotype; Haplotypes; Humans; Interleukin-8 - genetics; Malaysia; Male; Middle Aged; Models, Statistical; Odds Ratio; Polymorphism, Genetic; Risk; Software; 多态性; 大肠癌; 易感性; 白细胞介素; 等位基因频率; 风险; 马来西亚; Malaysia; Malaysians; Interleukin-8 -251T>A; Colorectal cancer; Polymorphism
• ISSN: 1007-9327; 2219-2840
• DOI: 10.3748/wjg.v18.i21.2668

AIM： To investigate the allele and genotype frequencies and associated risk of interleukin （IL）-8-251T〉A polymorphism on colorectal cancer （CRC） susceptibility risk. METHODS： Peripheral blood samples of 255 normal controls and 255 clinically and histopathologically confirmed CRC patients were genotyped for IL-8-251T〉A polymorphism employing allele-specific polymerase chain reaction. The relative association of variant allele and genotypes with CRC susceptibility risk was determined by calculating the odds ratios （ORs）. Corresponding χ 2 tests on the CRC patients and controls were carried out and 95% confidence intervals （CIs） were determined using Fisher＇s exact test. The allele frequencies and its risk association were calculated using FAMHAP, haplotype association analysis software. RESULTS： On comparing the frequencies of genotypesof patients and controls, the homozygous variant AA was significantly higher in CRC patients （P = 0.002） compared to controls. Investigation on the association of the polymorphic genotypes with CRC susceptibility risk, showed that the homozygous variant IL-8 -251AA had a significantly increased risk with OR 3.600 （95% CI： 1.550-8.481, P = 0.001）. In the case of allele frequencies, variant allele A of IL-8 -251 showed a significantly increased risk of CRC predisposition with OR 1.32 （95% CI： 1.03-1.69,P = 0.003）. CONCLUSION： Variant allele and genotype of IL-8 （-251 T〉A） was significantly associated with CRC susceptibility risk and could be considered as a high-risk variant for CRC predisposition.