Polychlorinated biphenyls are not substrates for the multidrug resistance transporter-1

• Published in: Toxicology and applied pharmacology Vol. 187; no. 3; pp. 168 - 177
• Main Authors: Tampal, Nilufer M, Robertson, Larry W, Srinivasan, Cidambi, Ludewig, Gabriele
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 15.03.2003; Elsevier
• Subjects: Adenosine Triphosphatases - metabolism; Animals; ATPase; Biological and medical sciences; Biological Transport, Active; Blood brain barrier; Brain - metabolism; Cells, Cultured; Chemical and industrial products toxicology. Toxic occupational diseases; Humans; Kidney - cytology; Kidney - metabolism; MDR; mdr1a knock-out mice; Medical sciences; Mice; Mice, Knockout; Molecular Structure; Multidrug Resistance-Associated Proteins - genetics; Multidrug Resistance-Associated Proteins - metabolism; Organ Specificity; PCB77; Polychlorinated biphenyls; Polychlorinated Biphenyls - metabolism; Substrate Specificity; Swine; Time Factors; Toxicology; Transport; Various organic compounds; ATPase; mdr1a knock-out mice; Polychlorinated biphenyls; PCB77; MDR; Transport; Blood brain barrier; Biological transport; Rodentia; P Glycoprotein; mdrla knock-out mice; Polychlorobiphenyl; Vertebrata; Mammalia; Mouse; Animal; Multiple resistance
• ISSN: 0041-008X; 1096-0333
• DOI: 10.1016/S0041-008X(02)00069-8

The multidrug resistance (MDR) transporter is a phosphorylated glycoprotein (P-gp) that has been implicated in the efflux of a large variety of xenobiotics, thereby protecting vital organs. This study examines the hypothesis that the multidrug resistance transporter is involved in restricting the entry of polychlorinated biphenyls (PCBs) into the brain. Three test systems were used. First, the ATPase activity of the human P-gp was measured as an indicator of the interaction of PCBs with the MDR transporter. PCB congeners and metabolites included in the study were PCB 153, PCB 169, PCB 77, and the 4-hydroxy and 4,4′-dihydroxy metabolites of PCB 77. An increase in ATPase activity was observed for all the PCBs tested except the 4-hydroxy metabolite of PCB 77. Second, we studied the transport of 14C-PCB 77 and 14C-PCB153 in a cell-culture model using porcine kidney cells expressing the human MDR1 or the mouse mdr1a gene and compared it to the transport in control cells. No difference in directional transport due to P-gp was observed with either of the congeners in any of the cell lines. Finally, the distribution pattern of 14C-PCB 77 in mdr1a knockout mice and genetically matched wild-type mice was measured. No significant differences in tissue distribution, especially in the brain tissue, were observed between wild-type and mdr1a knockout mice. These results suggest that some PCB congeners can bind to the MDR1 transporter; however, they may not be transported by it.