MicroRNA-891b is an independent prognostic factor of pancreatic cancer by targeting Cbl-b to suppress the growth of pancreatic cancer cells

• Published in: Oncotarget Vol. 7; no. 50; pp. 82338 - 82353
• Main Authors: Dong, Qian, Li, Ce, Che, Xiaofang, Qu, Jinglei, Fan, Yibo, Li, Xiaohan, Li, Yue, Wang, Qian, Liu, Yunpeng, Yang, Xianghong, Qu, Xiujuan
• Format: Journal Article
• Language: English
• Published: United States Impact Journals LLC 13.12.2016
• Subjects: 3' Untranslated Regions; Adaptor Proteins, Signal Transducing - genetics; Adaptor Proteins, Signal Transducing - metabolism; Animals; Binding Sites; Biomarkers, Tumor - genetics; Biomarkers, Tumor - metabolism; Carcinoma, Pancreatic Ductal - genetics; Carcinoma, Pancreatic Ductal - metabolism; Carcinoma, Pancreatic Ductal - pathology; Carcinoma, Pancreatic Ductal - surgery; Cell Line, Tumor; Cell Proliferation; Cyclin-Dependent Kinase Inhibitor p21 - genetics; Cyclin-Dependent Kinase Inhibitor p21 - metabolism; Female; Gene Expression Profiling - methods; Gene Expression Regulation, Neoplastic; Humans; Kaplan-Meier Estimate; Male; Mice, Inbred BALB C; Mice, Nude; MicroRNAs - genetics; MicroRNAs - metabolism; Middle Aged; Oligonucleotide Array Sequence Analysis; Pancreatic Neoplasms - genetics; Pancreatic Neoplasms - metabolism; Pancreatic Neoplasms - pathology; Pancreatic Neoplasms - surgery; Proto-Oncogene Proteins c-cbl - genetics; Proto-Oncogene Proteins c-cbl - metabolism; Research Paper; RNA Interference; Signal Transduction; Smad3 Protein - genetics; Smad3 Protein - metabolism; Time Factors; Transfection; microRNA-891b (miR-891b); pancreatic ductal adenocarcinoma (PDAC); Cbl-b; prognosis; overall survival (OS)
• ISSN: 1949-2553; 1949-2553
• DOI: 10.18632/oncotarget.11001

Growing evidence has revealed that microRNAs could regulate the proliferation of pancreatic ductal adenocarcinoma (PDAC) cells and predict the prognosis of PDAC. Here the comparative microRNA expression profiles of the good and poor prognosis groups were performed by microRNA microarray. MicroRNA-891b (miR-891b) was screened and validated to be a prognostic predictor of PDAC in the initial group and further evaluated to be an independent predictor for the overall survival of resectable PDACs in an independent cohort. By a series of cellular and animal experiments, as well as clinical specimen analyses, miR-891b was confirmed to target the Cbl-b gene, promot the expression of tumor suppressor p21 protein and inhibit the proliferation of PDAC cells. The results provide a theoretical basis for the study of miR-891b as an independent prognostic predictor of PDAC and the role of miR-891b/Cbl-b pathway in this prediction, as well as the identification of new targets for PDAC.