A preparative small-molecule mimic of liver CYP450 enzymes in the aliphatic C-H oxidation of carbocyclic N -heterocycles

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 120; no. 29; p. e2300315120
• Main Authors: Chambers, Rachel K, Weaver, Jacob D, Kim, Jinho, Hoar, Jason L, Krska, Shane W, White, M Christina
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 18.07.2023
• Subjects: Aliphatic compounds; Antipsychotics; Buspirone; Catalysis; Catalysts; Celecoxib; COX-2 inhibitors; Cytochrome; Cytochrome P-450 Enzyme System - chemistry; Cytochrome P450; Cytochromes P450; Drug development; Drugs; Enzymes; Fragments; Fungicides; Liver; Metabolism; Metabolites; Microsomes; Microsomes, Liver; Nitrogen; Oxidation; Oxidation-Reduction; Penconazole; Pharmaceutical Preparations - chemistry; Pharmaceuticals; Physical Sciences; RNA-directed RNA polymerase; Substrates; CYP450 mimic; oxidation; C–H activation; metabolism; bioisosteres
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2300315120

An emerging trend in small-molecule pharmaceuticals, generally composed of nitrogen heterocycles ( -heterocycles), is the incorporation of aliphatic fragments. Derivatization of the aliphatic fragments to improve drug properties or identify metabolites often requires lengthy de novo syntheses. Cytochrome P450 (CYP450) enzymes are capable of direct site- and chemo-selective oxidation of a broad range of substrates but are not preparative. A chemoinformatic analysis underscored limited structural diversity of -heterocyclic substrates oxidized using chemical methods relative to pharmaceutical chemical space. Here, we describe a preparative chemical method for direct aliphatic oxidation that tolerates a wide range of nitrogen functionality (chemoselective) and matches the site of oxidation (site-selective) of liver CYP450 enzymes. Commercial small-molecule catalyst Mn(CF -PDP) selectively effects direct methylene oxidation in compounds bearing 25 distinct heterocycles including 14 out of 27 of the most frequent -heterocycles found in U.S. Food and Drug Administration (FDA)-approved drugs. Mn(CF -PDP) oxidations of carbocyclic bioisostere drug candidates (for example, HCV NS5B and COX-2 inhibitors including valdecoxib and celecoxib derivatives) and precursors of antipsychotic drugs blonanserin, buspirone, and tiospirone and the fungicide penconazole are demonstrated to match the major site of aliphatic metabolism obtained with liver microsomes. Oxidations are demonstrated at low Mn(CF -PDP) loadings (2.5 to 5 mol%) on gram scales of substrate to furnish preparative amounts of oxidized products. A chemoinformatic analysis supports that Mn(CF -PDP) significantly expands the pharmaceutical chemical space accessible to small-molecule C-H oxidation catalysis.