Novel thin layer flow-cell screen-printed graphene electrode for enzymatic sensors

• Published in: Biosensors & bioelectronics Vol. 93; pp. 298 - 304
• Main Authors: Kanso, Hussein, González García, María Begoña, Llano, Laura Fernández, Ma, Su, Ludwig, Roland, Fanjul Bolado, Pablo, Santos, David Hernández
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 15.07.2017
• Subjects: Ascomycota - enzymology; Biosensing Techniques; Calibration; Carbohydrate Dehydrogenases - chemistry; Carbohydrate Dehydrogenases - isolation & purification; Cellobiose dehydrogenase; Direct electron transfer; Electrodes; Flow injection analysis; Graphene nanomaterials; Graphite - chemistry; Lactose - chemistry; Photocrosslinkable poly(vinyl alcohol) bearing styrylpyridinium groups (PVA-SbQ); Polyvinyls - chemistry; Printing; Thin layer flow-cell screen-printed electrode; Thin layer flow-cell screen-printed electrode; Cellobiose dehydrogenase; Photocrosslinkable poly(vinyl alcohol) bearing styrylpyridinium groups (PVA-SbQ); Direct electron transfer; Flow injection analysis; Graphene nanomaterials
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2016.08.069

A new Screen-printed electrodes (SPE) integrated in one channel flow-cell was developed. The one channel flow-cell is attached and directly changeable with electrode. In the new flow-cell the injection is done through an “in-line luer injection port” which can be less aggressive than wall-jet flow cell for a biological recognition element immobilized on the surface of the electrode. The sample volume can be easily controlled by the operator through a syringe. In this novel thin layer flow-cell screen-printed electrodes, the working electrode was modified with graphene materials, and an enhancement of electroactive area to 388% over a standard electrode was found. This new configuration was applied to study the entrapped cellobiose dehydrogenase from the ascomycete Corynascus thermophilus (CtCDH) in a photocrosslinkable PVA-based polymer. The calibration curve of lactose using optimized parameters shows a wide linear measurement ranges between 0.25 and 5mM. A good operational stability of the CtCDH-PVA-modified graphene electrode is obtained, which keeps the same initial activity during 8h and exhibits a good storage stability with a decrease of only 9% in analytical response after 3 months storage at 4◦C. [Display omitted] •An original flow-cell where channel slide is directly mounted over the screen-printed electrodes plat-form was proposed.•The modification of the new electrode with graphene materials was also studied, and an enhancement of electroactive area up to 388% was achieved.•The new configuration was applied for investigation of the entrapped cellobiose dehydrogenase (CtCDH) in photocrosslinkable polymer (PVA-SbQ).•CtCDH in PVA-SbQ exhibits a good storage stability with a decrease of only 9% in analytical response after 3 months storage at 4°C.