Sensitive Chemiluminescent Detection of Nucleosides and Their Derivatives Using the Material Exchange of Chemically Copper(II)-Modified Natural Zeolite

• Published in: Analytical Sciences Vol. 8; no. 5; pp. 647 - 653
• Main Authors: OHNO, Yoichi, HOBO, Toshiyuki, TAMURA, Risako, ISHII, Mikita
• Format: Journal Article
• Language: English
• Published: Tokyo The Japan Society for Analytical Chemistry 01.10.1992; Japan Society for Analytical Chemistry; Japan Science and Technology Agency
• Subjects: Analytical chemistry; Chemiluminescent detection; Chemistry; cupric ion; Exact sciences and technology; flow injection analysis; material exchange; Miscellaneous; nucleoside; zeolite; Chemical modification; Chemical analysis; Detection limit; Nucleoside; Chemiluminescence; Copper III Ions; Zeolite; Ion exchange; Flow injection; Modified material
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.8.647

The sensitivity for the chemiluminescent (CL) detection of nucleosides and their derivatives was improved by using clinoptirolite chemically modified with cupric nitrate, Cu-Zeo. Cu-Zeo plays a key role as a solid medium for such so-called material exchangers as ion exchange between copper(II) coated on Cu-Zeo and nucleosides and their derivatives. The properties for material exchange were examined by a batch method in which Cu-Zeo(1.5g; particle size, 250mesh) was added into the analyte solution (100ml). Analytes were indirectly determined by the CL analysis of cupric ions eluted from the Cu-Zeo through the material exchange. For the CL detection of cupric ions, an FIA/CL system using a 1, 10-phenanthroline CL was employed. The lower limits of detection for analytes were improved by 42 times for adenosine (1.9pmol/20μl) and by 16 times for uridine-5′-diphosphate (5.5pmol/20μl), compared with those by our previous method.