Sensitive Chemiluminescent Detection of Nucleosides and Their Derivatives Using the Material Exchange of Chemically Copper(II)-Modified Natural Zeolite
The sensitivity for the chemiluminescent (CL) detection of nucleosides and their derivatives was improved by using clinoptirolite chemically modified with cupric nitrate, Cu-Zeo. Cu-Zeo plays a key role as a solid medium for such so-called material exchangers as ion exchange between copper(II) coate...
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Published in | Analytical Sciences Vol. 8; no. 5; pp. 647 - 653 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Tokyo
The Japan Society for Analytical Chemistry
01.10.1992
Japan Society for Analytical Chemistry Japan Science and Technology Agency |
Subjects | |
Online Access | Get full text |
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Summary: | The sensitivity for the chemiluminescent (CL) detection of nucleosides and their derivatives was improved by using clinoptirolite chemically modified with cupric nitrate, Cu-Zeo. Cu-Zeo plays a key role as a solid medium for such so-called material exchangers as ion exchange between copper(II) coated on Cu-Zeo and nucleosides and their derivatives. The properties for material exchange were examined by a batch method in which Cu-Zeo(1.5g; particle size, 250mesh) was added into the analyte solution (100ml). Analytes were indirectly determined by the CL analysis of cupric ions eluted from the Cu-Zeo through the material exchange. For the CL detection of cupric ions, an FIA/CL system using a 1, 10-phenanthroline CL was employed. The lower limits of detection for analytes were improved by 42 times for adenosine (1.9pmol/20μl) and by 16 times for uridine-5′-diphosphate (5.5pmol/20μl), compared with those by our previous method. |
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ISSN: | 0910-6340 1348-2246 |
DOI: | 10.2116/analsci.8.647 |