Influence of transfected SV40 early region on growth and differentiation of human endothelial cells

• Published in: Journal of biotechnology Vol. 25; no. 3; pp. 349 - 356
• Main Authors: Hohenwarter, O., Zinser, E., Schmatz, C., Rüker, F., Katinger, H.
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 01.09.1992; Amsterdam Elsevier; New York, NY
• Subjects: Antigens, Polyomavirus Transforming - analysis; Biological and medical sciences; Biotechnology; Cell Differentiation; Cell Division; Cell Transformation, Viral; Cells, Cultured; DNA transfection; Endothelium, Vascular - cytology; Fundamental and applied biological sciences. Psychology; Genetic engineering; Genetic technics; Human endothelial cells; Humans; Large T antigen; Methods. Procedures. Technologies; Miscellaneous; Simian virus 40 - genetics; SV40 virus; Synthetic digonucleotides and genes. Sequencing; Transfection; von Willebrand factor; von Willebrand Factor - analysis; Human endothelial cells; Large T antigen; DNA transfection; SV40 virus; von Willebrand factor; Human; Endothelial cell; Polyomavirus; Papovaviridae; Cell differentiation; Gene expression; Virus; Transfection; DNA; Detection; Willebrand factor; Immunofluorescence; T antigen; Growth factor
• ISSN: 0168-1656; 1873-4863
• DOI: 10.1016/0168-1656(92)90167-8

Endothelial cells isolated from human umbilical veins show a limited in vitro life span of about 40 population doublings. Cell division is dependent on the presence of endothelial cell growth factor in the culture medium. We have transfected primary endothelial cells with a plasmid containing the early region of SV40 virus. Large T positive cells were obtained which grew in the absence of endothelial cell growth factor at low serum concentrations and showed a prolonged lifespan. Expression of von Willebrand factor and SV40 large T antigen was detected simultaneously in transfected cells.