Genotoxic effect of ozone in human peripheral blood leukocytes

• Published in: Mutation research Vol. 517; no. 1; pp. 13 - 20
• Main Authors: Dı́az-Llera, Silvia, González-Hernández, Yanela, Prieto-González, E.A, Azoy, Angel
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 27.05.2002; Elsevier
• Subjects: Biological and medical sciences; Catalase; Catalase - therapeutic use; Cells, Cultured; Comet Assay; DNA Damage; Dose-Response Relationship, Drug; Drug toxicity and drugs side effects treatment; Human leukocytes; Humans; Leukocytes - metabolism; Medical sciences; Miscellaneous (drug allergy, mutagens, teratogens...); Oxygen - pharmacology; Ozone; Ozone - pharmacology; Pharmacology. Drug treatments; SCGE; SCGE; Ozone; Catalase; Human leukocytes; DNA damage; Comet assay; Human; Enzyme; Leukocyte; Mutagenicity testing; Toxicity; Prevention; Gases; Peroxidases; Oxidoreductases; Lesion
• ISSN: 1383-5718; 0027-5107; 1879-3592
• DOI: 10.1016/S1383-5718(02)00022-0

The genotoxic effect of ozone was studied in human leukocytes in vitro, using the single cell gel electrophoresis (SCGE) assay. Cell treatment for 1 h at 37 °C with 0.9–5.3 mM O 3 resulted in a dose-dependent increase of DNA damage, comparable to that induced by 4–40 mM of H 2O 2, used as a positive control. This effect of ozone was reversed by post-treatment incubation of the cells for 45–90 min at 37 °C, and prevented by pre-incubation of the cells with catalase (20 μg/ml). These results demonstrate that O 3 induces DNA-damage in primary human leukocytes. The damage is rapidly repaired, and probably mediated by the formation of H 2O 2.