Structural Dynamics of Chondrocytes during Culturing

We performed comparative analysis of the morphology of chondrocytes in normal cartilage, after their isolation from the tissue, and at different stages of culturing; structural dynamics of cells during culturing was also studied. Significant morphological differences in chondrocytes at the specified...

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Published inBulletin of experimental biology and medicine Vol. 164; no. 2; pp. 274 - 280
Main Authors Omelyanenko, N. P., Rodionov, S. A.
Format Journal Article
LanguageEnglish
Published New York Springer US 01.12.2017
Springer
Springer Nature B.V
Subjects
Biomedical and Life Sciences
Biomedicine
Cartilage
Cell Biology
Chondrocytes
Comparative analysis
Cytoplasm
Internal Medicine
Laboratory Medicine
Microscopy
Pathology
Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
Transmission electron microscopy
structural dynamics
cell culturing
chondrocytes
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Summary:We performed comparative analysis of the morphology of chondrocytes in normal cartilage, after their isolation from the tissue, and at different stages of culturing; structural dynamics of cells during culturing was also studied. Significant morphological differences in chondrocytes at the specified stages of their preparation to in vivo use were revealed. Pronounced structural changes (blebbing and cytoplasm swelling) were found in chondrocytes before their implantation, which can affect the formation of cartilage regenerate. The study was performed using light microscopy methods including time-lapse recording of the cell cultures with differential interference Nomarski contrasting combined with transmission electron microscopy.
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ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-017-3972-y