Effectiveness of peptone-yeast extract (P-Y) medium in the cultivation and isolation of Entamoeba histolytica/Entamoeba dispar in Turkish patients

• Published in: Diagnostic microbiology and infectious disease Vol. 45; no. 2; pp. 127 - 130
• Main Authors: Dagci, Hande, Balcioglu, I.Cuneyt, Ertabaklar, Hatice, Kurt, Ozgur, Atambay, Metin
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.02.2003; Elsevier
• Subjects: Amibiasis; Animals; Biological and medical sciences; Cell Extracts; Cells, Cultured; Culture; Culture Media; Dobell-Laidlaw; Entamoeba - classification; Entamoeba - growth & development; Entamoeba - isolation & purification; Entamoeba dispar; Entamoeba histolytica; Entamoeba histolytica - growth & development; Entamoeba histolytica - isolation & purification; Entamoebiasis - microbiology; Human protozoal diseases; Humans; Infectious diseases; Medical sciences; P-Y medium; Parasitic diseases; Peptones - chemistry; Protozoal diseases; Robinson; Time Factors; Turkey; Yeasts - cytology; Turkey; P-Y medium; Dobell-Laidlaw; Robinson; Culture; Performance evaluation; Human; Culture medium; Protozoa; Protozoal disease; Lobosea; Entamoeba histolytica; Parasitosis; Method; Infection; Microorganism culture; Isolation; Amebiasis; Diagnosis; Feces
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/S0732-8893(02)00508-4

Amebiasis is a common protozoan infection worldwide, causing serious health problems in both children and adults. Today, almost 10% of the world population is infected with Entamoeba histolytica/Entamoeba dispar. The aims of this study were both the comparison of the reproduction rates and densities of E. histolytica/E. dispar in Robinson, Dobell-Laidlaw and P-Y culture media and isolation of E. histolytica/E. dispar from stool samples in Peptone-Yeast (P-Y) medium. Trophozoites and cysts of E. histolytica/E. dispar, maintained in Robinson medium, and stool samples of patients with amebiasis were inoculated into P-Y, Robinson and Dobell-Laidlaw culture media. Reproduction rates reached their peak levels 48 h after the inoculation in all culture media. Reproduction rates in P-Y and Robinson media were found similar; however, they were higher than the reproduction rate in Dobell-Laidlaw medium ( p < 0.01); there was no statistically significant difference between the reproduction rates of P-Y and Robinson media ( p > 0.05). Twelve isolates from 12 patients were cultivated in P-Y medium and checked for reproduction everyday for 7 days. Twelve of the 12 (100%) isolates were cultivated in P-Y medium, indicating that the P-Y was an effective medium for the isolation of E. histolytica/E. dispar in stool samples. According to these results, P-Y medium could be preferred in immunologic, serologic and molecular studies and, thus the definitive diagnosis of amebiasis due to its low cost and simple formula.