Production and utilization of catalase using Saccharomyces cerevisiae

A culture medium was designed for maximal production of biomass and intracellular catalase by a potent enriched culture of Saccharomyces cerevisiae CFR-201. Growth conditions for catalase production were optimal at 30°C for 72 h, in shake flasks on a rotary shaker (200 rpm). The effect of various ca...

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Bibliographic Details
Published inProcess biochemistry (1991) Vol. 34; no. 2; pp. 187 - 191
Main Authors Venkateshwaran, G., Somashekar, D., Prakash, M.H., Agrawal, Renu, Basappa, S.C., Joseph, Richard
Format Journal Article
LanguageEnglish
Published Elsevier Ltd 01.02.1999
Subjects
Catalase
Cold pasteurization
H2O2
Saccharomyces
Wheat bran extract
Saccharomyces
Catalase
H2O2
Wheat bran extract
Cold pasteurization
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Summary:A culture medium was designed for maximal production of biomass and intracellular catalase by a potent enriched culture of Saccharomyces cerevisiae CFR-201. Growth conditions for catalase production were optimal at 30°C for 72 h, in shake flasks on a rotary shaker (200 rpm). The effect of various carbon and nitrogen sources on catalase production were also studied. S. cerevisiae CFR-201 produced five times more intracellular catalase than a local baker’s yeast strain. The cell free extract used as source of catalase removed 99·9% residual H2O2 from cold pasteurized milk at 45°C for 1 h.
ISSN:1359-5113
1873-3298
DOI:10.1016/S0032-9592(98)00087-9