Auto-aggregation and co-aggregation ability in bifidobacteria and clostridia

• Published in: Folia microbiologica Vol. 53; no. 3; pp. 263 - 269
• Main Authors: Vlkova, E.,Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Mikrobiologie, Vyzivy a Dietetiky, Rada, V.,Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Mikrobiologie, Vyzivy a Dietetiky, Smehilova, M.,Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Mikrobiologie, Vyzivy a Dietetiky, Killer, J.,Ceska Zemedelska Univ., Prague (Czech Republic). Katedra Mikrobiologie, Vyzivy a Dietetiky
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 2008; Springer Nature B.V
• Subjects: AISLAMIENTO; Animals; Applied Microbiology; Babies; Bacteria; Bacterial Adhesion; BIFIDOBACTERIUM; Bifidobacterium - classification; Bifidobacterium - growth & development; Bifidobacterium - isolation & purification; Bifidobacterium - physiology; Biomedical and Life Sciences; CALVES; Carbon - metabolism; Carbon sources; Cattle; CLOSTRIDIO; CLOSTRIDIUM; Clostridium - growth & development; Clostridium - isolation & purification; Clostridium - physiology; Culture Media; Digestive system; Environmental Engineering/Biotechnology; FAECES; FECES; Feces - microbiology; FENOTIPOS; GENERO HUMANO; GENRE HUMAIN; HECES; HIDROFOBICIDAD; http://www.fao.org/aos/agrovoc#c_1219; http://www.fao.org/aos/agrovoc#c_1680; http://www.fao.org/aos/agrovoc#c_23898; http://www.fao.org/aos/agrovoc#c_2772; http://www.fao.org/aos/agrovoc#c_33502; http://www.fao.org/aos/agrovoc#c_37744; http://www.fao.org/aos/agrovoc#c_4586; http://www.fao.org/aos/agrovoc#c_4810; http://www.fao.org/aos/agrovoc#c_5776; Humans; Hydrophobic and Hydrophilic Interactions; HYDROPHOBICITE; HYDROPHOBICITY; Immunology; Intestines - microbiology; ISOLATION; ISOLEMENT; Life Sciences; MANKIND; Microbiology; MICROSCOPIA; MICROSCOPIE; MICROSCOPY; PHENOTYPE; PHENOTYPES; Probiotics; Surface Properties; TERNERO; VEAU; Xylene; Probiotic Strain; Intestinal Mucus; Probiotic Bacterium; Cell Surface Hydrophobicity; Lactic Acid Bacterium
• ISSN: 0015-5632; 1874-9356
• DOI: 10.1007/s12223-008-0040-z

A total of 142 human and 88 calf bifidobacteria were isolated and identified; about 12% of all isolated strains exhibited auto-aggregation (Agg) phenotype (Agg+). Properties considered to be predicting for their adhesion to intestine, i.e. auto-aggregation, and hydrophobicity were determined by xylene extraction in 18 human and 8 calf origin bifidobacteria. Co-aggregation of 8 human bifidobacteria with 8 clostridia was also evaluated. Agg varied between 16.3 and 96.4%, hydrophobicity values ranged from 0 to 82.8%. The strongest Agg and hydrophobicity were observed in B. bifidum and B. merycicum isolates. However, there were no statistically significant correlations between these two properties. Variability in the percentage of Agg and hydrophobicity was observed after cultivation of bifidobacteria on different carbon sources. All bifidobacteria showed co-aggregation ability with clostridia tested but there were remarkable differences depending on specific combinations of strains. The bifidobacterial strains with the highest ability to co-aggregate with clostridia were B. bifidum I4 and B. longum I10 isolated from infants; these strains gave also high values of Agg. Agg properties together with co-aggregation ability with potential pathogen can be used for preliminary selection of probiotic bacteria.