Extended-spectrum beta-lactamase (ESBL)- and non-ESBL producing Escherichia coli surveillance in surface water sources in Edo State, Nigeria: a public health concern

• Published in: Scientific reports Vol. 14; no. 1; pp. 21658 - 13
• Main Authors: Beshiru, Abeni, Isokpehi, Nnenna A., Igbinosa, Isoken H., Akinnibosun, Olajide, Ogofure, Abraham G., Igbinosa, Etinosa O.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 17.09.2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/326/22/1434; 631/326/41/2535; Ampicillin; Anti-Bacterial Agents - pharmacology; Antimicrobial agents; Antimicrobial resistance; beta-Lactamases - genetics; beta-Lactamases - metabolism; Cefotaxime; Ceftazidime; Ceftriaxone; Drug Resistance, Multiple, Bacterial - genetics; E coli; Ertapenem; Escherichia coli; Escherichia coli - drug effects; Escherichia coli - enzymology; Escherichia coli - genetics; Escherichia coli - isolation & purification; Health risks; Humanities and Social Sciences; Humans; Imipenem; Meropenem; Microbial Sensitivity Tests; multidisciplinary; Multidrug resistance; Nigeria - epidemiology; Nitrofurantoin; Polymerase chain reaction; Public awareness; Public Health; Science; Science (multidisciplinary); Seasonal variations; Selective media; Surface water; Water Microbiology; β Lactamase; Nigeria
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-72993-w

This research explores the antimicrobial resistance (AMR) profiles and prevalence of extended-spectrum beta-lactamase (ESBL) and non-ESBL-producing Escherichia coli in Ojerame Dam and Ovokoto Spring, Edo State, Nigeria. Over 12 months, water was systematically sampled to accommodate seasonal variations and analyzed by employing an ESBL-selective medium for bacterial species. Additionally, bacterial isolates underwent identification and characterization using polymerase chain reaction (PCR) and disk diffusion methods to evaluate their susceptibility to antimicrobials. Results indicated significant prevalence of ESBL-producing E. coli , which exhibited complete resistance to common antimicrobials like ceftriaxone, ceftazidime, cefotaxime, and ampicillin while demonstrating 100% sensitivity to ertapenem, imipenem, meropenem, and nitrofurantoin. Non-ESBL-producing E. coli were resistant to ampicillin but sensitive to other antimicrobials mentioned earlier. Furthermore, both ESBL and non-ESBL-producing E. coli displayed multidrug resistance to varying degrees. Specific ESBL genes, including bla TEM , bla CTX-M-1 , and bla CTX-M-15 , were identified, alongside resistance genes like tetA , tetM , sul1 , sul2 , sul3 , qnrA , qnrB , and qnrS in E. coli. This study pioneers the documentation of ESBL-producing E. coli in surface water in the region. This signals impending health risks associated with water being a reservoir of resistant genes while emphasizing the urgency for further research and public awareness concerning the quality of surface water.