Identification and quantification of constituents of Gardenia jasminoides Ellis (Zhizi) by HPLC-DAD–ESI–MS

• Published in: Food chemistry Vol. 134; no. 2; pp. 1199 - 1204
• Main Authors: Bergonzi, M.C., Righeschi, C., Isacchi, B., Bilia, A.R.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 15.09.2012; Elsevier
• Subjects: Biological and medical sciences; Caffeoyl quinic acid derivatives; Chromatography, High Pressure Liquid - methods; Crocins; detection limit; Drugs, Chinese Herbal - chemistry; Food industries; Fundamental and applied biological sciences. Psychology; Gardenia - chemistry; Gardenia jasminoides; Gardenia jasminoides Ellis (Zizhi); high performance liquid chromatography; HPLC-DAD–MS; iridoid glycosides; Iridoids; methanol; Method validation; qualitative analysis; Qualitative and quantitative analysis; quantitative analysis; quinic acid; Spectrometry, Mass, Electrospray Ionization - instrumentation; Spectrometry, Mass, Electrospray Ionization - methods; wavelengths; HPLC-DAD–MS; Iridoids; Method validation; Gardenia jasminoides Ellis (Zizhi); Qualitative and quantitative analysis; Caffeoyl quinic acid derivatives; Crocins; Iridoid; Validation; HPLC-DAD-MS; Gardenia; Crocin; HPLC chromatography; Identification; Method; Dicotyledones; Angiospermae; Rubiaceae; Spermatophyta; Qualitative analysis; Mass spectrometry; Constituent; Quantitative analysis
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2012.02.157

► A HPLC method was developed for identification of compounds in Gardenia jasminoides. ► Twelve compounds were identified including iridoids, caffeoyl quinic acid derivatives and crocins. ► The analytical method was validated. ► This assay was used for characterisation of five commercial samples. A simple, rapid and specific HPLC method was carried out for the analysis of characteristic constituents in Gardenia jasminoides Ellis (Zhizi), namely iridoids, caffeoyl quinic acid derivatives and crocins. The separation was successfully obtained using a C18 column by gradient elution with mixtures of methanol and water as mobile phases; detection wavelength was set at 240nm for iridoid glycosides, 315nm for quinic acid derivatives and 438nm for crocins. The analytical method was validated and the quantification of active compounds, namely iridoids, was performed. Linearity, precision, repeatability, stability, accuracy, limit of detection (LOD) and limit of quantification (LOQ) were also reported. This assay was successfully applied for qualitative and quantitative analysis of five commercial samples of G. jasminoides Ellis.