Opposite effects of GPR120 and GPR40 on cell motile activity induced by ethionine in liver epithelial cells

• Published in: Biochemical and biophysical research communications Vol. 456; no. 1; pp. 135 - 138
• Main Authors: Ishii, Shuhei, Hirane, Miku, Kato, Sayumi, Fukushima, Nobuyuki, Tsujiuchi, Toshifumi
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 02.01.2015
• Subjects: Animals; Cell motility; Cell Movement; Cell Proliferation; Epithelial Cells - metabolism; Ethionine; Ethionine - chemistry; GPR120; GPR40; Liver - metabolism; Liver cell; Male; Polymerase Chain Reaction; Rats; Rats, Inbred F344; Receptors, G-Protein-Coupled - metabolism; Cell motility; RT; GPR120; Liver cell; GPCR; GPR40; PCR; Ethionine
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2014.11.047

•Gpr120 and Gpr40 expressions were elevated by ethionine in WB-F344 cells.•Ethionine stimulated cell motile activity of WB-F344 cells.•GPR120 knockdown suppressed the cell motile activity by ethionine.•The cell motile activity was enhanced by GPR40 antagonist GW1100.•Opposite effects of GPR120 and GPR40 are involved in the cell motile activity induced by ethionine. Free fatty acids (FFAs) are dietary nutrients which act as ligands for FFAs receptors. G-protein-coupled receptor 120 (GPR120) and GPR40 are activated by long and medium chain FFAs. In the present study, we investigated the role of the GPR120 and GPR40 in cell motile activity stimulated by ethionine in rat liver epithelial WB-F344 cells. Cells were treated with ethionine at a concentration of 10μM every 24h for 2days. The expression levels of the Gpr120 and Gpr40 genes in WB-F344 cells treated ethionine were significantly higher than those in untreated cells. In cell motility assay, the cell motile activity of WB-F344 cells was markedly elevated by ethionine, compared with untreated cells. To evaluate the effects of GPR120 on the cell motile activity by ethionine, we established GPR120 knockdown cells from WB-F344 cells. The cell motile activity stimulated by ethionine was significantly suppressed by GPR120 knockdown. In addition, a potent GPR40 antagonist GW1100 enhanced the cell motile activity by ethionine. These results suggest that opposite effects of GPR120 and GPR40 may be involved in the cell motile activity stimulated by ethionine in WB-F344 cells.