The role of conventional antibodies targeting the CD4 binding site and CD4-induced epitopes in the control of HIV-1 CRF01_AE viruses

• Published in: Biochemical and biophysical research communications Vol. 508; no. 1; pp. 46 - 51
• Main Authors: Thida, Win, Kuwata, Takeo, Maeda, Yosuke, Yamashiro, Tetsu, Tran, Giang Van, Nguyen, Kinh Van, Takiguchi, Masafumi, Gatanaga, Hiroyuki, Tanaka, Kazuki, Matsushita, Shuzo
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.01.2019
• Subjects: Antibody; CRF01_AE; Fc-mediated signaling; HIV-1; Neutralization; HIV-1; Fc-mediated signaling; Antibody; CRF01_AE; Neutralization
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2018.11.063

HIV-1 CRF01_AE viruses are highly prevalent in Southeast Asia. However, vulnerability sites in Env of CRF01_AE viruses have not been investigated sufficiently. We examined the sensitivity of CRF01_AE viruses from Japan and Vietnam, together with subtype B viruses from Japan, to neutralization and Fc-mediated signaling. Neutralization coverage of broadly neutralizing antibodies (bnAbs), 2G12 and b12, was significantly low against CRF01_AE viruses, compared with subtype B viruses. In contrast, the conventional antibody targeting the CD4 binding site (CD4bs), 49G2, showed better neutralization and Fc-mediated signaling activities against CRF01_AE viruses than subtype B viruses. Fc-mediated signaling activity of anti-CD4 induced (CD4i) antibody, 4E9C, was also detected against CRF01_AE viruses more than subtype B viruses. These results suggest that conventional antibodies against CD4bs and CD4i may play an important role in the control of CRF01_AE viruses. •Compared with subtype B, CRF01_AE viruses were resistant to bnAbs, 2G12 and b12.•Abs to CD4bs and CD4i are relatively effective against CRF01_AE viruses.•Conventional Abs may play an important role in the control of CRF01_AE viruses.