Hyperoxia caused by microdialysis perfusion decreased striatal monoamines: involvement of oxidative stress
Due to complex influence, such as utilization and permeability of arterial vessels to oxygen, there is a considerable difference of oxygen tension between extracellular fluid and perfusate usually used in microdialysis (30–60 Torr versus 145 Torr). Dialysate dopamine and monoamine metabolites—3,4-di...
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Published in | Neurochemistry international Vol. 42; no. 6; pp. 465 - 470 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Oxford
Elsevier Ltd
01.05.2003
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Due to complex influence, such as utilization and permeability of arterial vessels to oxygen, there is a considerable difference of oxygen tension between extracellular fluid and perfusate usually used in microdialysis (30–60
Torr versus 145
Torr). Dialysate dopamine and monoamine metabolites—3,4-dihydroxyphenylacetic acid, homovanillic acid and 5-hydroxyindoleacetic acid were measured under different kinds of oxygen tension solutions (145, 72, 48
Torr). In the acute and anesthetized group, dopamine, 3,4-dihydroxyphenylacetic acid, 5-hydroxyindoleacetic acid and homovanillic acid increased 72, 93, 86 and 65%, respectively when changing the perfusate from 145
Torr to near physiological 48
Torr, while in chronic and conscious group, carried out 72
h after surgery, these compounds showed obscure increases (only homovanillic acid produced a significant change of 14%).
The different effect of perfusate oxygen tension on dialysate levels of monoamines in anesthetized and conscious rats might be caused by oxidative stress triggered by hyperoxia combined with anesthesia and surgical trauma. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0197-0186 1872-9754 |
DOI: | 10.1016/S0197-0186(02)00159-6 |