Intravenous toxicity of fructose-1,6-bisphosphate in rats

• Published in: Toxicology letters Vol. 143; no. 1; pp. 73 - 81
• Main Authors: Nunes, Fernanda Bordignon, Gaspareto, Patrick Barcelos, Vianna Santos, Roberto Christ, de Assis, Márcio, Graziottin, Cássio Meyer, Biolchi, Vanderlei, Alves Filho, José Carlos Farias, Lunardelli, Adroaldo, Ávila, Luı́s D., Pires, Melissa Guerra Simões, Wächter, Paulo Harald, De Oliveira, Jarbas Rodrigues
• Format: Journal Article
• Language: English
• Published: Shannon Elsevier Ireland Ltd 05.06.2003; Amsterdam Elsevier Science
• Subjects: Animals; Biological and medical sciences; Calcium - blood; Drug toxicity and drugs side effects treatment; Electrocardiography - drug effects; Fructose-1,6-bispbosphate; Fructosediphosphates - administration & dosage; Fructosediphosphates - toxicity; Injections, Intravenous; LD 50; Lethal Dose 50; Male; Medical sciences; Pharmacology. Drug treatments; Phosphates - blood; PROBIT; Rats; Rats, Wistar; Toxicity; Toxicity: cardiovascular system; PROBIT; Toxicity; LD 50; Fructose-1,6-bispbosphate; Cardiocirculatory arrest; Intravenous administration; LD50PROBIT; Rat; Ketose; Rodentia; Cardiovascular disease; Glycosyl phosphate; Vertebrata; Mammalia; Lethal dose 50; Animal; Organic phosphate
• ISSN: 0378-4274; 1879-3169
• DOI: 10.1016/S0378-4274(03)00075-4

Fructose-1,6-bisphosphate (FBP) is a bisphosphorilated sugar with a protective action against events that lead to cellular damage. The toxicity of the drug was assessed when administered intravenously in Wistar rats in doses of between 250 and 4000 mg/kg. Ionic calcium, total calcium, inorganic serum phosphate and the electrocardiographic profile of these animals were assessed. The lethal dose (LD 50) was established by means of PROBIT processing. There was no reduction in the levels of total calcium, with the administration of increased doses of FBP, although there was a significant reduction in the levels of ionic calcium in those groups that received 250 mg/kg and over. The serum phosphate showed a significant statistical increase in those groups that received 750 mg/kg and over. The LD 50 obtained in 24 h was 1068 mg/kg. Though it was not possible to elucidate the toxic mechanism of FBP, the electrocardiogram (ECG) showed that all the rats died of cardiac arrest.